Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CAT5, A NEW GENE NECESSARY FOR DEREPRESSION OF GLUCONEOGENIC ENZYMES IN SACCHAROMYCES-CEREVISIAE
Autore:
PROFT M; KOTTER P; HEDGES D; BOJUNGA N; ENTIAN KD;
Indirizzi:
UNIV FRANKFURT,BIOZENTRUM NIEDERURSEL,INST MIKROBIOL D-60439 FRANKFURT GERMANY UNIV FRANKFURT,BIOZENTRUM NIEDERURSEL,INST MIKROBIOL D-60439 FRANKFURT GERMANY
Titolo Testata:
EMBO journal
fascicolo: 24, volume: 14, anno: 1995,
pagine: 6116 - 6126
SICI:
0261-4189(1995)14:24<6116:CANGNF>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARBON CATABOLITE REPRESSION; GLUCOSE REPRESSION; PROTEIN-KINASE; PHOSPHOENOLPYRUVATE CARBOXYKINASE; ESCHERICHIA-COLI; HEXOKINASE PII; MIG1 REPRESSOR; LACZ FUSIONS; YEAST; MUTANTS;
Keywords:
CAT5; GLUCONEOGENESIS; GLUCOSE REPRESSION; PCK1; SACCHAROMYCES CEREVISIAE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
56
Recensione:
Indirizzi per estratti:
Citazione:
M. Proft et al., "CAT5, A NEW GENE NECESSARY FOR DEREPRESSION OF GLUCONEOGENIC ENZYMES IN SACCHAROMYCES-CEREVISIAE", EMBO journal, 14(24), 1995, pp. 6116-6126

Abstract

PCK1 encoding phosphoenolpyruvate carboxykinase is transcriptionally regulated by two upstream activating elements, By screening for mutants that failed to derepress a UAS2(PCK1)-CYC1-lacZ reporter gene we isolated the new recessive derepression mutation cat5. The CAT5 gene encodes a protein of 272 amino acids showing a 42% identity to the ZC395.2gene product of Caenorhabditis elegans whose function is unknown, Deletion of CAT5 caused a complete loss of glucose derepression affectinggluconeogenic key enzymes, Respiration, but not mitochondrial cytochrome c oxidase activity, was also affected. CAT5 expression is 5- to 6-fold repressed by glucose, and CAT5 transcriptional activation was dependent on CAT1 (SNF1), CAT8 and CAT5 itself. The CAT5 gene is necessary for UAS1(PCK1) and UAS2(PCK1) protein binding since a carbon source-specific interaction was no longer detectable in cat5 mutants, Glucosederepression of gluconeogenesis depends on the active Cat1 (Snf1) protein kinase and the Cat8 zinc cluster activator. Mig1p-independent overexpression of CAT8 did not stimulate activation of gluconeogenic promoters in cat1 and in cat5 mutants, Since Cat8p multicopy expression suppresses the ethanol growth deficiency in cat1 (snf1) mutants, these results indicate that activation of Cat8p by the Cat1p (Snf1p) kinase and the Cat5p protein might be necessary for release from glucose repression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 06:43:07