Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DETECTION OF STRAIN-SPECIFIC ANTIGENIC EPITOPES ON THE LIPO-OLIGOSACCHARIDE OF HAEMOPHILUS-PARASUIS BY USE OF MONOCLONAL AND POLYCLONAL ANTIBODIES
Autore:
ZUCKER BA; BAGHIAN A; TRUAX R; OREILLY KL; STORZ J;
Indirizzi:
LOUISIANA STATE UNIV,SCH VET MED,DEPT VET MICROBIOL & PARASITOL BATONROUGE LA 70803 LOUISIANA STATE UNIV,SCH VET MED,DEPT VET MICROBIOL & PARASITOL BATONROUGE LA 70803
Titolo Testata:
American journal of veterinary research
fascicolo: 1, volume: 57, anno: 1996,
pagine: 63 - 67
SICI:
0002-9645(1996)57:1<63:DOSAEO>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
INFLUENZAE TYPE-B; HEMOPHILUS-INFLUENZAE; GLASSERS-DISEASE; NEISSERIA-GONORRHOEAE; POLYACRYLAMIDE GELS; VIRULENCE; LIPOOLIGOSACCHARIDES; LIPOPOLYSACCHARIDE; EXPRESSION; SWINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
B.A. Zucker et al., "DETECTION OF STRAIN-SPECIFIC ANTIGENIC EPITOPES ON THE LIPO-OLIGOSACCHARIDE OF HAEMOPHILUS-PARASUIS BY USE OF MONOCLONAL AND POLYCLONAL ANTIBODIES", American journal of veterinary research, 57(1), 1996, pp. 63-67

Abstract

Objective-To investigate the antigenic diversity of lipooligosaccharides of Haemophilus parasuis. Procedures-Immunoblot assays were done with monoclonal and polyclonal antibodies on whole-cell lysates. individual colonies of H parasuis strains H 54, H 53, and H 128 were tested for reactivity with lipo-oligosaccharide-specific monoclonal antibodiesafter a single passage on chocolate agar, and colonies of strain H 54were analyzed after 10 passages. Colony blot tests were used to screen H parasuis strains for spontaneously occurring antigenic variation in their lipo-oligosaccharides. Results-Eight H parasuis strains were separated into 4 lipo-oligosaccharide serovars on the basis of immunoblot reactions with 3 polyclonal rabbit antisera. Nine monoclonal antibodies against lipo-oligosaccharides of a lipo-oligosaccharide-serovar Istrain reacted with all tested serovar I strains but failed to react with other H parasuis strains. Conclusions-Variations in the antigenicreactivity after 1 or 10 passages on chocolate agar were not observed. The serovar I lipo-oligosaccharide strains included virulent as wellas avirulent H parasuis strains, indicating that these epitopes do not correlate directly with virulence properties of H parasuis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 20:46:37