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Titolo:
ANALYSIS OF CORD-BLOOD CD34(+) CELLS PURIFIED AFTER CRYOPRESERVATION
Autore:
NICOL A; NIEDA M; DONALDSON C; DENNINGKENDALL P; BRADLEY B; HOWS J;
Indirizzi:
SOUTHMEAD GEN HOSP,MED SCH UNIT,STEM CELL RES LAB,SOUTHMEAD RD BRISTOL BS10 5NB AVON ENGLAND UNIV BRISTOL,DEPT TRANSPLANTAT SCI,STEM CELL RES LAB BRISTOL AVON ENGLAND
Titolo Testata:
Experimental hematology
fascicolo: 14, volume: 23, anno: 1995,
pagine: 1589 - 1594
SICI:
0301-472X(1995)23:14<1589:AOCCCP>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM; PROGENITOR CELLS; INVITRO; RECONSTITUTION; GROWTH;
Keywords:
LONG-TERM CULTURE-INITIATING CELLS; CD34(+) CELL; CRYOPRESERVATION; CORD BLOOD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
A. Nicol et al., "ANALYSIS OF CORD-BLOOD CD34(+) CELLS PURIFIED AFTER CRYOPRESERVATION", Experimental hematology, 23(14), 1995, pp. 1589-1594

Abstract

Many practical issues regarding processing blood samples for cord blood banking remain. After cryopreservation, a reduction in clonogenicity has been reported, although it is unknown whether this is associatedwith lower potential for long-term engraftment. CD34(+) cell purification of cryopreserved cord blood (CB) may be important for the clinical application of in vitro expansion. We compared purity, yield, clonogenicity, and growth in long-term stromal-based culture of fresh and cryopreserved CD34(+) purified cells (n = 12) using the miniMACS(R) separation system. Mean purity of CD34(+) cells was 93% when processed before and 73% when processed after cryopreservation. Fresh CD34(+) cellshad higher clonogenic potential than cryopreserved cells (45 vs 20%, p < 0.05) in CFU-Mix assays, indicating that progenitor cell loss during cryopreservation is due in part to reduced cloning efficiency of viable CD34(+) cells. In long-term culture (LTC) on irradiated normal human bone marrow stroma (n = 7), CFU-GM production in the two groups was the same over 12 weeks, suggesting identical long-term culture-initiating cell (LTC-IC) numbers. We conclude that apparent clonogenic cellloss during cryopreservation is associated with relative sparing of the more primitive LTC-ICs. CFU-Mix assays may therefore underestimate the transplant potential of cryopreserved CB. Purification of CD34(+) cells following cryopreservation gives sufficient purity for detailed evaluation of CD34(+) cells and for stem cell expansion.

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Documento generato il 18/09/20 alle ore 11:06:48