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Titolo:
EFFECTS OF DIFFERENT NITROGEN-SOURCES ON NITROGEN-FIXATION AND BACTERIAL-GROWTH OF PANTOEA AGGLOMERANS AND AZOSPIRILLUM SP IN BACTERIAL PURE CULTURE - AN INVESTIGATION USING N-15(2) INCORPORATION AND ACETYLENE-REDUCTION MEASURES
Autore:
RUPPEL S; MERBACH W;
Indirizzi:
GROSSBEEREN ERFURT EV,INST GEMUSE & ZIERPFLANZENBAU,THEODOR ECHTERMEYER WEG 1 D-14979 GROSSBEEREN GERMANY
Titolo Testata:
Microbiological research
fascicolo: 4, volume: 150, anno: 1995,
pagine: 409 - 418
SICI:
0944-5013(1995)150:4<409:EODNON>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Keywords:
ASSOCIATIVE DINITROGEN FIXATION; ACETYLENE REDUCTION ACTIVITY; N-15(2) INCORPORATION; NITROGEN TOLERANCE OF NITROGENASE; BACTERIAL GROWTH EFFICIENCY AZOSPIRILLUM SP; PANTOEA AGGLOMERANS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
S. Ruppel e W. Merbach, "EFFECTS OF DIFFERENT NITROGEN-SOURCES ON NITROGEN-FIXATION AND BACTERIAL-GROWTH OF PANTOEA AGGLOMERANS AND AZOSPIRILLUM SP IN BACTERIAL PURE CULTURE - AN INVESTIGATION USING N-15(2) INCORPORATION AND ACETYLENE-REDUCTION MEASURES", Microbiological research, 150(4), 1995, pp. 409-418

Abstract

Due to energetic limitations the microbiological associative nitrogenfixation from the air is solely not able to cover the nitrogen demandof the plant. It is therefore necessary to find diazotrophic strains which are able to fur atmospheric nitrogen in presence of additional nitrogen sources and to excrete nitrogen into the surrounding medium. The diazotrophic strains Azospirillum sp. and Pantoea agglomerans were proved to fix Nz in presence of ammonium, nitrate, glutamine and glutamate in nutrient broth as sole nitrogen sources. Azospirillum sp. was completely inhibited in its nitrogenase activity after addition of 1 mM glutamine, 1 mM KNO3 and 2 mM NH4Cl and higher concentrations of these nitrogen sources. The total inhibition of nitrogenase activity of Azospirillum sp. induced by 50 mg NH4+ - N1(-1) was derepressed by simultaneous addition of L-Methionine-DL-sulfoximine (MSX) the inhibitor of glutamine synthetase (GS). Pantoea agglomerans, however, showed a different response to ammonium, nitrate, glutamine and glutamic acid application than Azospirillum sp. The ammonium induced partial repression of nitrogenase activity was not derepressed by addition of MSX. Nitrogenase activity of P. agglomerans was stronger inhibited by glutamic acid than by ammonium or glutamine and was uneffected by nitrate. The nitrogenase activity analysed by acetylene reduction was compared to the amount of N-15(2) incorporated into the bacterial biomass followingincubation with N-15(2) labelled air. The good correlation (r = 0.81) of acetylene reduction activity and the amount of biologically fixednitrogen (estimated by N-15(2) incorporation measures with Azospirillum spp.) remains stable after the addition of 50 mg NO3- - N1(-1) or 50 mg NH4+ - N1(-1). These results allowed us to use the acetylene reduction activity as a measure of biological nitrogen fixation by associative bacterial strains independent on the nitrogen source applied.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:28:46