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Titolo:
COMPARISON OF HUMAN HEPATOCYTES ISOLATED FROM LIVERS ACCEPTED OR DISCARDED FOR ORTHOTOPIC TRANSPLANTATION
Autore:
GROOTHUIS GMM; SANDKER GW; PRUIM J; WEERT B; SLOOFF MJH; MEIJER DKF;
Indirizzi:
UNIV GRONINGEN,CTR PHARM,DEPT PHARMACOKINET & DRUG DELIVERY 9713 AW GRONINGEN NETHERLANDS UNIV GRONINGEN HOSP,DEPT POSITRON EMISS TOMOG,GUIDE 9713 EZ GRONINGENNETHERLANDS UNIV GRONINGEN HOSP,DEPT SURG 9713 EZ GRONINGEN NETHERLANDS
Titolo Testata:
Toxicology in vitro
fascicolo: 6, volume: 9, anno: 1995,
pagine: 951 - 958
SICI:
0887-2333(1995)9:6<951:COHHIF>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISOLATED RAT HEPATOCYTES; ADULT HEPATOCYTES; DRUG TRANSPORT; CELL VIABILITY; DONOR LIVERS; UW SOLUTION; CULTURE; DEHYDROGENASE; PRESERVATION; METABOLISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
G.M.M. Groothuis et al., "COMPARISON OF HUMAN HEPATOCYTES ISOLATED FROM LIVERS ACCEPTED OR DISCARDED FOR ORTHOTOPIC TRANSPLANTATION", Toxicology in vitro, 9(6), 1995, pp. 951-958

Abstract

The aim of this study was to compare human hepatocytes isolated from livers accepted and from livers discarded for transplantation with respect to viability and drug transport function. In addition, the influence of age of the donor and preservation time of the liver on cell viability was determined. Cell viability was assessed by trypan blue exclusion, MTT reduction, morphological integrity and ATP content, and drug transport function by uptake and excretion of taurocholic acid. Hepatocytes could be isolated successfully from livers accepted as well asfrom livers discarded for transplantation, with a median yield of 5.0x 10(6) cells/g (range 0.1 to 42.4) and 0.7 x 10(6) cells/g (range 0.0 to 22.7), respectively (not significantly different). These cells were not significantly different with respect to viability and transportrate of taurocholate. Neither the age of the donor nor the duration of liver preservation (6-43 hr in University of Wisconsin solution) significantly influenced cell yield and viability. It is concluded that because of this overlap in cell viability, hepatocytes isolated both from accepted and from discarded livers can in principle be used to investigate drug transport functions in the human liver.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 13:43:25