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Titolo:
SALICYLATE-ENHANCED EXPOSURE OF KLEBSIELLA-PNEUMONIAE SUBCAPSULAR COMPONENTS
Autore:
SALO RJ; DOMENICO P; TOMAS JM; STRAUS DC; MERINO S; BENEDI VJ; CUNHA BA;
Indirizzi:
NASSAU CTY MED CTR,DEPT PEDIAT,2201 HEMPSTEAD TURNPIKE E MEADOW NY 11554 WINTHROP UNIV HOSP,DIV INFECT DIS MINEOLA NY 11501 UNIV BARCELONA,FAC BIOL,DEPT MICROBIOL E-08028 BARCELONA SPAIN TEXAS TECH UNIV,HLTH SCI CTR,DEPT MICROBIOL & IMMUNOL LUBBOCK TX 79430
Titolo Testata:
Infection
fascicolo: 6, volume: 23, anno: 1995,
pagine: 371 - 377
SICI:
0300-8126(1995)23:6<371:SEOKSC>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
GRAM-NEGATIVE BACTERIA; CAPSULAR POLYSACCHARIDE; O-ANTIGEN; ESCHERICHIA-COLI; SURFACE EXPOSURE; RESISTANCE; STRAINS; LIPOPOLYSACCHARIDE; OPSONOPHAGOCYTOSIS; INFECTIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
R.J. Salo et al., "SALICYLATE-ENHANCED EXPOSURE OF KLEBSIELLA-PNEUMONIAE SUBCAPSULAR COMPONENTS", Infection, 23(6), 1995, pp. 371-377

Abstract

The capsular polysaccharide (CPS) of Klebsiella pneumoniae is an important virulence factor. Salicylate, which inhibits CPS production, wasused to expose subcapsular antigens and components that may play an important role in host defense. Salicylate treatment greatly increased phagocytosis of five 01 serotypes by human polymorphonuclear leukocytes,vith normal rabbit serum and rabbit antisera against purified 01 lipopolysaccharide (01LPS) as opsonins (p<0.01 or <0.05). Similar resultswere obtained with rabbit antiserum against a non-encapsulated isogenic strain. To further determine how salicylate increases susceptibility to phagocytosis, the binding of monoclonal antibodies against 01LPS or the LPS core and the binding of complement component C3b were measured by ELISA. The data indicate that salicylate reduced the barrier ofCPS in serotypes 01:K1, 01:K10, and 01:K16 and unmasked subcapsular antigenic components in serotypes 01:K2 and 01:K66 so that bound opsonins could react with receptors on phagocytes. Serum bactericidal assayssupported this conclusion. Therefore, decapsulating agents such as salicylate accentuate phagocytosis of K. pneumoniae by making subcapsular antigens and components accessible to immune and nonimmune host defences and vaccination with subcapsular antigens may exhibit optimal protection against lethal infection when combined with salicylate therapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/10/20 alle ore 01:56:14