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Titolo:
EXOGENOUS MYELIN BASIC-PROTEIN PROMOTES OLIGODENDROCYTE DEATH VIA INCREASED CALCIUM INFLUX
Autore:
TZENG SF; DEIBLER GE; DEVRIES GH;
Indirizzi:
EDWARD HINES JR VET ADM HOSP,BLDG 1,ROOM D400J,5TH AVE & ROOSEVELT RDHINES IL 60141 VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT BIOCHEM & MOLEC BIOPHYS RICHMOND VA 23298 NIMH,CEREBRAL METAB LAB BETHESDA MD 20892
Titolo Testata:
Journal of neuroscience research
fascicolo: 6, volume: 42, anno: 1995,
pagine: 768 - 774
SICI:
0360-4012(1995)42:6<768:EMBPOD>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVOUS-SYSTEM; CELL-SURVIVAL; GANGLIOSIDES; PROLIFERATION; ASTROCYTES; TISSUE;
Keywords:
MYELIN BASIC PROTEIN; OLIGODENDROCYTES; GANGLIOSIDE GM1; CALCIUM INFLUX; CELL DEATH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
S.F. Tzeng et al., "EXOGENOUS MYELIN BASIC-PROTEIN PROMOTES OLIGODENDROCYTE DEATH VIA INCREASED CALCIUM INFLUX", Journal of neuroscience research, 42(6), 1995, pp. 768-774

Abstract

Treatment of cultured oligodendrocytes (OLGs) with micromolar quantities of myelin basic protein (MBP) caused a rapid, MBP-dose-dependent cell death, In contrast, a 72-hr incubation of OLGs with MBP peptides (1-44, 47-87, 88-151, or 152-167) at comparable concentrations had no effect on cell viability, MBP and MBP peptides (1-44 and 88-151) have been shown to interact with ganglioside GM1 (Tzeng et al.: J Neurochem Res: 42:758-767, 1995), This interaction has been reported to increasecalcium influx, Therefore, using the fluorescent dye Indo-1 and an ACAS laser cytometer, we examined the level of intracellular calcium in OLGs after MBP treatment, MBP was shown to provoke a rapid, dramatic, and sustained rise of intracellular calcium in most OLGs, The levels of elevated intracellular calcium were sustained and did not return to baseline even after 10 min, This increase of intracellular calcium wassuppressed in the presence of EGTA, indicating that the [Ca2+](i) rise was due to the entry of extracellular calcium, Incubation of cultured OLGs with MBP peptides (1-44 or 88-151) caused a modest and transitory elevation of intracellular calcium ions in a lower percentage of OLGs, The potent OLG cytotoxicity of intact MBP and the loss of potency after proteolysis raise the possibility that MBP proteolysis during demyelination protects OLGs from death. (C) 1995 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 15:54:57