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Titolo:
MOLECULAR PHYLOGENY BASED ON THE KAPPA-CASEIN AND CYTOCHROME-B SEQUENCES IN THE MAMMALIAN SUBORDER RUMINANTIA
Autore:
CHIKUNI K; MORI Y; TABATA T; SAITO M; MONMA M; KOSUGIYAMA M;
Indirizzi:
NATL INST ANIM IND,TSUKUBA NOURINDANCHI POB 5 TSUKUBA IBARAKI 305 JAPAN IBARAKI UNIV,FAC AGR AMI IBARAKI 30003 JAPAN NATL FOOD RES INST TSUKUBA IBARAKI 305 JAPAN
Titolo Testata:
Journal of molecular evolution
fascicolo: 6, volume: 41, anno: 1995,
pagine: 859 - 866
SICI:
0022-2844(1995)41:6<859:MPBOTK>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
MITOCHONDRIAL-DNA EVOLUTION; NUCLEOTIDE-SEQUENCE; RAPID EVOLUTION; GENOME; GENE; CDNA; AMPLIFICATION; ORGANIZATION; VERTEBRATES; LEVEL;
Keywords:
EVOLUTION; KAPPA-CASEIN; CYTOCHROME B; ARTIODACTYLA; RUMINANTIA; CAPRINAE; CAPRICORNIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
K. Chikuni et al., "MOLECULAR PHYLOGENY BASED ON THE KAPPA-CASEIN AND CYTOCHROME-B SEQUENCES IN THE MAMMALIAN SUBORDER RUMINANTIA", Journal of molecular evolution, 41(6), 1995, pp. 859-866

Abstract

Nucleotide sequences for the kappa-casein precursor proteins have been determined from the genomic DNAs or hair roots of the Ruminantia. The coding regions, exons 2, 3, and 4, were amplified separately via thethree kinds of PCRs and then directly sequenced. The primers were designed from the sequence of bovine kappa-casein gene; they were applicable for the amplification of the kappa-casein genes from the 13 species in the Ruminantia except exon 2 of the lesser mouse deer. These results permitted an easy phylogenetic analysis based on the sequences of an autosomal gene. A phylogenetic tree was constructed from the maturekappa-casein sequences and compared with the tree of the cytochrome bgenes which were sequenced from the same individuals. The Cervidae (sika deer, Cervus nippon) were separated from the branch of the Bovidaeon the tree of kappa-casein genes with a relatively high confidence level of the bootstrap analysis, but included in the branch of the Bovidae on the tree of cytochrome b genes. The kappa-casein tree indicateda monophyly of the subfamily Caprinae, although the internal brancheswere uncertain in the Caprinae. The tree based on the nucleotide sequences of cytochrome b genes clearly showed the relationships of the closely related species in the genus Capricornis consisting of serow (C.smatorensis), Japanese serow (C. crispus), and Formosan serow (C. swinhoei). These results would be explained by the difference of resolving power between the Ic-casein and the cytochrome b sequences.

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Documento generato il 21/09/20 alle ore 03:16:39