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Titolo:
GLUCOSE-INDUCED ISLET HYPEREMIA IS MEDIATED BY NITRIC-OXIDE
Autore:
MOLDOVAN S; LIVINGSTON E; ZHANG RS; KLEINMAN R; GUTH P; BRUNICARDI FC;
Indirizzi:
BAYLOR COLL MED,DEPT SURG,1 BAYLOR PLAZA HOUSTON TX 77030 UNIV CALIF LOS ANGELES,MED CTR LOS ANGELES CA 90024 W LOS ANGELES VET AFFAIRS MED CTR,DEPT SURG LOS ANGELES CA 90073 W LOS ANGELES VET AFFAIRS MED CTR,DEPT MED LOS ANGELES CA 90073
Titolo Testata:
The American journal of surgery
fascicolo: 1, volume: 171, anno: 1996,
pagine: 16 - 20
SICI:
0002-9610(1996)171:1<16:GIHIMB>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-FLOW; SECRETION; RAT; PANCREAS; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
S. Moldovan et al., "GLUCOSE-INDUCED ISLET HYPEREMIA IS MEDIATED BY NITRIC-OXIDE", The American journal of surgery, 171(1), 1996, pp. 16-20

Abstract

PURPOSE: TO determine whether hyperglycemia affects pancreatic islet microcirculation in vivo and whether nitric oxide is a mediator. METHODS: Islet blood flow was measured before and after infusion of glucoseduring in vivo microscopy of mouse pancreatic islet. The pancreas of male BALB/c mice was exteriorized and viewed under the microscope utilizing monochromatic transmitted light. The carotid artery and tail vein were cannulated and systemic blood pressure was monitored continuously. Under fluorescent light, a 0.02 mt bolus of 2% fluorescein isothyocyanate (FITC-albumin) was injected intra-arterially and the first pulse of FITC-albumin through an islet capillary was videorecorded. Following equilibration, either glucose or normal saline 300 mg/g of body weight was given intravenously. Five minutes later, a second bolus was given and the second pulse was videorecorded. The study was repeated in the presence of N omega-nitro-L-arginine methyl ester (L-NAME). The FITC-albumin bolus mean transit time (TT) and observed cross time (OCT) through the islet were calculated using slow-motion video analysis of the recorded images. RESULTS: Infusion of glucose resulted in a significant increase in islet blood flow with no change in systemic blood pressure: baseline TT was 20 +/- 1.3 pixel/0.03 sec and baseline OCT was 0.6 +/- 0.04 seconds; during hyperglycemia, TT was 16.1 +/- 1 pixel/0.03 sec, and OCT was 0.48 +/- 0.03 seconds (n = 11, P <0.05 versus basal via paired t-test). Continuous infusion of L-NAME negated the effect of hyperglycemia on islet blood flow: baseline TT was 20 +/- 1.8 pixel/0.03 sec and OCT was and 0.6 +/- 0.05 seconds; during hyperglycemia, TT was 20 +/- 1.1 pixel/0.03 sec and OCT was 0.6 +/- 0.33 seconds (n = 10; P <0.05 versus glucose via unpaired t-test). CONCLUSIONS: These data suggest that hyperglycemia results in increased islet capillary flow and nitric oxide is a regulator of islet blood flow during in vivo microscopy of the mouse islet.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 01:01:54