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Titolo:
NEW ANALYSIS METHOD FOR PROTEIN PHOSPHATASE TYPE 2A INHIBITORS USING THE FIREFLY BIOLUMINESCENCE SYSTEM
Autore:
ISOBE M; SUGIYAMA Y; ITO T; OHTANI II; TOYA Y; NISHIGOHRI Y; TAKAI A;
Indirizzi:
NAGOYA UNIV,SCH AGR SCI,ORGAN CHEM LAB,CHIKUSA KU NAGOYA AICHI 46401 JAPAN NAGOYA UNIV,SCH MED,DEPT PHYS,SHOWA KU NAGOYA AICHI 466 JAPAN
Titolo Testata:
Bioscience, biotechnology, and biochemistry
fascicolo: 12, volume: 59, anno: 1995,
pagine: 2235 - 2238
SICI:
0916-8451(1995)59:12<2235:NAMFPP>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MARINE TOXIC POLYETHERS; SPONGE DISCODERMIA-CALYX; OKADAIC ACID; C-SEGMENT; ELUCIDATION; METABOLITES; DERIVATIVES; TAUTOMYCIN; LUCIFERIN; POTENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
M. Isobe et al., "NEW ANALYSIS METHOD FOR PROTEIN PHOSPHATASE TYPE 2A INHIBITORS USING THE FIREFLY BIOLUMINESCENCE SYSTEM", Bioscience, biotechnology, and biochemistry, 59(12), 1995, pp. 2235-2238

Abstract

A new analysis method for protein phosphatase type 2A inhibitors was established that uses the firefly bioluminescence system for detection. Thus, firefly luciferin phosphate was used as a substrate, and the liberated free luciferin was determined from the amount of light emitted from the immobilized luciferase. This method was successfully used to determine the activities of known inhibitors, i.e., okadaic acid, calyculin A, microcystin-LR and tautomycin using less than 10 pmol of a sample.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 06:42:59