Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
IDENTIFICATION AND PURIFICATION OF A BOVINE LIVER MITOCHONDRIAL NAD(-GLYCOHYDROLASE())
Autore:
ZHANG J; ZIEGLER M; SCHNEIDER R; KLOCKER H; AUER B; SCHWEIGER M;
Indirizzi:
INNSBRUCK UNIV,INST BIOCHEM,PETER MAYR STR 1A A-6020 INNSBRUCK AUSTRIA INNSBRUCK UNIV,INST BIOCHEM A-6020 INNSBRUCK AUSTRIA FREE UNIV BERLIN,INST BIOCHEM W-1000 BERLIN GERMANY INNSBRUCK UNIV,DEPT UROL A-6020 INNSBRUCK AUSTRIA
Titolo Testata:
FEBS letters
fascicolo: 3, volume: 377, anno: 1995,
pagine: 530 - 534
SICI:
0014-5793(1995)377:3<530:IAPOAB>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PYRIDINE-NUCLEOTIDES; NAD+ GLYCOHYDROLASE; ADP-RIBOSYLATION; CALCIUM; RELEASE; PROTEINS;
Keywords:
NAD(+)-GLYCOHYDROLASE; ADPR-ARGININE HYDROLASE; MITOCHONDRIAL MEMBRANE; PURIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
J. Zhang et al., "IDENTIFICATION AND PURIFICATION OF A BOVINE LIVER MITOCHONDRIAL NAD(-GLYCOHYDROLASE())", FEBS letters, 377(3), 1995, pp. 530-534

Abstract

Nonenzymatic ADP-ribosylation of mitochondrial proteins is thought toplay a role in the regulation of Ca2+ efflux from mitochondria. It has been shown that intramitochondrial ADP-ribose is generated by a specific NAD(+)glycohydrolase, which catalizes hydrolysis of NAD(+) to ADP-ribose and nicotinamide, We purified this enzyme from bovine liver mitochondrial membranes. The final preparation had a 1660-fold purified enzyme activity and contained a main protein band with an apparent molar mass of 32,000 in a SDS-polyacrylamide gel. The identity of this protein band with NAD(+)-glycohydrolase was verified by renaturation of its enzymatic activity, Partial amino acid sequence information was obtained from two enzyme fragments after proteolytic cleavage of the protein band in the SDS-polyacrylamide gel. Searches in protein databasesrevealed that an arginine ADP-ribosyl hydrolase harbours two stretches of amino acids that are highly similar to the partial NAD(+)-glycohydrolase sequences.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/05/20 alle ore 09:08:35