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Titolo:
IDENTIFICATION OF MULTIPLE PEPC ISOGENES IN LEAVES OF THE FACULTATIVECRASSULACEAN ACID METABOLISM (CAM) PLANT KALANCHOE-BLOSSFELDIANA-POELLN CV TOM-THUMB
Autore:
GEHRIG H; TAYBI T; KLUGE M; BRULFERT J;
Indirizzi:
TH DARMSTADT,INST BOT,SCHNITTSPAHNSTR 10 D-64287 DARMSTADT GERMANY UNIV PARIS 11,INST BIOTECHNOL PLANTES,CNRS 1128,URA F-91405 ORSAY FRANCE
Titolo Testata:
FEBS letters
fascicolo: 3, volume: 377, anno: 1995,
pagine: 399 - 402
SICI:
0014-5793(1995)377:3<399:IOMPII>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOSPHOENOLPYRUVATE CARBOXYLASE; INDUCTION; EVOLUTION; SEQUENCE;
Keywords:
CRASSULACEAN ACID METABOLISM (CAM); GENE FAMILY; KALANCHOE; PHOSPHOENOLPYRUVATE CARBOXYLASE; PHYLOGENETIC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
H. Gehrig et al., "IDENTIFICATION OF MULTIPLE PEPC ISOGENES IN LEAVES OF THE FACULTATIVECRASSULACEAN ACID METABOLISM (CAM) PLANT KALANCHOE-BLOSSFELDIANA-POELLN CV TOM-THUMB", FEBS letters, 377(3), 1995, pp. 399-402

Abstract

In the facultative Crassulacean Acid Metabolism (CAM) plant Kalanchoeblossfeldiana cv, Tom Thumb, CAM can be induced by short-day treatment or water deficiency stress, From young leaves of well-watered and water-stressed individuals of this plant, cDNA clones coding for a partial sequence of the key enzyme of CAM, phosphoenolpyruvate carboxylase,were isolated after transcription of mRNA. cDNA polymorphism was established by enzyme restriction profiles and sequencing data. Four PEPC isogenes could be shown to exist in K. blossfeldiana forming two gene pairs, with 95%-98% homology inside and only 75% between the pairs. One cDNA sequence pair having a length of 1113 bp and an open reading frame of 371 AA was identified as PEPC isoform specific for the C-3 state, whereas the pair having a length of 1116 bp and an open reading frame of 372 AA could be attributed to the CAM state, These results were confirmed by Southern Blot hybridization. (EMBL, accession numbers X 87818, X 87819, X 87820, X 87821.)

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 14:55:43