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Titolo:
ANTIGENIC PROPERTIES AND DIAGNOSTIC POTENTIAL OF PUUMALA-VIRUS NUCLEOCAPSID PROTEIN EXPRESSED IN INSECT CELLS
Autore:
VAPALAHTI O; LUNDKVIST A; KALLIOKOKKO H; PAUKKU K; JULKUNEN I; LANKINEN H; VAHERI A;
Indirizzi:
UNIV HELSINKI,HAARTMAN INST,DEPT VIROL,POB 21 SF-00014 HELSINKI FINLAND NATL PUBL HLTH INST SF-00300 HELSINKI FINLAND SWEDISH INST INFECT DIS CONTROL S-10521 STOCKHOLM SWEDEN
Titolo Testata:
Journal of clinical microbiology
fascicolo: 1, volume: 34, anno: 1996,
pagine: 119 - 125
SICI:
0095-1137(1996)34:1<119:APADPO>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED-IMMUNOSORBENT-ASSAY; S-GENOME SEGMENTS; STRAIN HALLNAS B1; NEPHROPATHIA-EPIDEMICA; HEMORRHAGIC-FEVER; RENAL SYNDROME; HANTAAN VIRUS; BACULOVIRUS EXPRESSION; MONOCLONAL-ANTIBODIES; ETIOLOGIC AGENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
O. Vapalahti et al., "ANTIGENIC PROPERTIES AND DIAGNOSTIC POTENTIAL OF PUUMALA-VIRUS NUCLEOCAPSID PROTEIN EXPRESSED IN INSECT CELLS", Journal of clinical microbiology, 34(1), 1996, pp. 119-125

Abstract

Puumala virus (PUU) is a member of the genus Hantavirus in the familyBunyaviridae and the causative agent of nephropathia epidemica, a European form of hemorrhagic fever with renal syndrome. Sera of nephropathia epidemica patients react specifically with PUU nucleocapsid (N) protein. In order to safely provide large quantities of antigen for diagnostic purposes, PUU Sotkamo strain N protein was expressed by using the baculovirus system in Sf9 insect cells to up to 30 to 50% of the total cellular protein. The recombinant N protein (bac-PUU-N) was solubilized with 6 M urea, dialyzed, and purified by anion exchange liquid chromatography. In an immunoglobulin M mu-capture assay purified and unpurified bac-PUU-N antigen showed identical results compared with the results of a similar assay based on native PUU antigen grown in Vero E6 cells. An immunoglobulin G monoclonal antibody-capture assay based on unpurified bac-PUU-N also showed results identical to those of an assay with native PUU-N antigen. Moreover, a panel of monoclonal antibodies reactive with eight different epitopes showed identical reactivitypatterns with both natural and bac-PUU-N antigen, while two epitopes in PUU-N expressed as a fusion protein in Escherichia coli were not recognized. Puumala hantavirus N protein expressed by the baculovirus system offers a safe and inexpensive source of specific antigen for large-scale diagnostic and seroepidemiological purposes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:53:48