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Titolo:
IDENTIFICATION OF PORPHYROMONAS-GINGIVALIS BY AN ELISA USING A CELLULOSE-ACETATE PAPER POINT
Autore:
YASUDA K; OGAWA T; SHIMAUCHI H;
Indirizzi:
OSAKA UNIV,FAC DENT,DEPT ORAL MICROBIOL,1-8 YAMADAOKA SUITA OSAKA 565JAPAN OSAKA UNIV,FAC DENT,DEPT ORAL MICROBIOL SUITA OSAKA 565 JAPAN
Titolo Testata:
Journal of microbiological methods
fascicolo: 1, volume: 24, anno: 1995,
pagine: 21 - 27
SICI:
0167-7012(1995)24:1<21:IOPBAE>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLACK-PIGMENTED BACTEROIDES; PERIODONTAL-DISEASES; SERUM IGG; CELLS; ASACCHAROLYTICUS; ANTIBODIES;
Keywords:
ELISA; PAPER POINT; PERIODONTAL DISEASES; PORPHYROMONAS GINGIVALIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
K. Yasuda et al., "IDENTIFICATION OF PORPHYROMONAS-GINGIVALIS BY AN ELISA USING A CELLULOSE-ACETATE PAPER POINT", Journal of microbiological methods, 24(1), 1995, pp. 21-27

Abstract

The ELISA testing method with a cellulose acetate paper point for detecting Porphyromonas gingivalis in subgingival plaque samples was investigated. Rabbit polyclonal IgG (Poly Pgf-I) and mouse monoclonal antibodies (mAbs Pgf-I) against fimbriae from P. gingivalis 381 were obtained. Poly Pgf-I and mAbs Pgf-I reacted with cell suspensions consisting of the range of 2x10(4) to 2x10(6) P. gingivalis 381 cells by the ELISA method. The developed paper point could be classified into four degrees, -, +, + + and + + + according to the number of P. gingivalis cells. Poly Pgf-I and mAbs Pgf-I reacted with P. gingivalis cells, whilst neither of these antibodies reacted with whole cells of other oral or non-oral black-pigmented bacterial species. Analysis by the ELISA method resulted in definite immunoreactivity in subgingival plaque samples of patients with adult periodontitis, whilst no detectable reactivity was found in those of normal healthy subjects. The simple and rapidELISA method using the paper point may be useful for the identification of P. gingivalis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:39:16