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Titolo:
EFFECTS OF ENHANCED LYSINE EPSILON-AMINOTRANSFERASE ACTIVITY ON CEPHAMYCIN BIOSYNTHESIS IN STREPTOMYCES-CLAVULIGERUS
Autore:
MALMBERG LH; HU WS; SHERMAN DH;
Indirizzi:
UNIV MINNESOTA,DEPT MICROBIOL,1479 CORTNER AVE,240 ST PAUL MN 55108 UNIV MINNESOTA,DEPT MICROBIOL ST PAUL MN 55108 UNIV MINNESOTA,INST BIOL PROC TECHNOL ST PAUL MN 55108 UNIV MINNESOTA,DEPT CHEM ENGN & MAT SCI MINNEAPOLIS MN 55455
Titolo Testata:
Applied microbiology and biotechnology
fascicolo: 1-2, volume: 44, anno: 1995,
pagine: 198 - 205
SICI:
0175-7598(1995)44:1-2<198:EOELEA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEPHALOSPORIUM-ACREMONIUM; ALPHA-AMINOADIPATE; C BIOSYNTHESIS; CATABOLISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
L.H. Malmberg et al., "EFFECTS OF ENHANCED LYSINE EPSILON-AMINOTRANSFERASE ACTIVITY ON CEPHAMYCIN BIOSYNTHESIS IN STREPTOMYCES-CLAVULIGERUS", Applied microbiology and biotechnology, 44(1-2), 1995, pp. 198-205

Abstract

A recombinant strain of S. clavuligerus (LHM100) that contains an additional copy of the gene (lar) encoding lysine epsilon-aminotransferase (LAT) was analyzed and compared to the wild-type for intracellular concentrations of primary metabolites involved in cephamycin C biosynthesis. This strain had been shown previously to produce higher levels of the antibiotic because of increased levels of LAT, a rate-limiting enzyme involved in the production of alpha-amino-adipic acid. The results showed that the overall growth kinetics of the two strains were comparable, including the intracellular concentrations of cysteine, valine and lysine. In contrast, 60% higher antibiotic production was observed in LHM100, which reflected a significant temporal variation in specific metabolite production rate. The time profile of LAT activity was consistently higher in LHM100, however, alpha-aminoadipic acid levels showed unexpected variation during the growth cycle. These results support the proposal that rate-limiting enzymes in cephamycin C biosynthesis are temporally controlled, and indicate that optimization of metabolite production will require differential overexpression of several biosynthetic genes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 06:03:59