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Titolo:
ENTEROCHELIN ACQUISITION IN CAMPYLOBACTER-COLI - CHARACTERIZATION OF COMPONENTS OF A BINDING-PROTEIN-DEPENDENT TRANSPORT-SYSTEM
Autore:
RICHARDSON PT; PARK SF;
Indirizzi:
AFRC,INST FOOD RES,READING LAB READING RG6 6BZ BERKS ENGLAND AFRC,INST FOOD RES,READING LAB READING RG6 6BZ BERKS ENGLAND
Titolo Testata:
Microbiology
, volume: 141, anno: 1995,
parte:, 12
pagine: 3181 - 3191
SICI:
1350-0872(1995)141:<3181:EAIC-C>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
GRAM-NEGATIVE BACTERIA; ESCHERICHIA-COLI; NUCLEOTIDE-SEQUENCES; IRON TRANSPORT; JEJUNI; DNA; SIDEROPHORES; CLONING; GENES;
Keywords:
CAMPYLOBACTER COLI; ENTEROCHELIN; LIPOPROTEIN; SIDEROPHORE; CEU;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
P.T. Richardson e S.F. Park, "ENTEROCHELIN ACQUISITION IN CAMPYLOBACTER-COLI - CHARACTERIZATION OF COMPONENTS OF A BINDING-PROTEIN-DEPENDENT TRANSPORT-SYSTEM", Microbiology, 141, 1995, pp. 3181-3191

Abstract

Siderophore-mediated iron uptake systems play a central rose in the pathogenesis of infection for many bacterial pathogens. Campylobacter species are not thought to produce siderophores, yet they are able to utilize both ferrichrome and enterochelin as sources of iron. Part of an operon named ceuBCDE, encoding components of a periplasmic binding-protein-dependent transport (PBT) system for the uptake of a ferric siderophore from Campylobacter coli, was cloned directly into Escherichiacoli using a plasmid rescue technique. Phenotypic and genetic analyses of this system showed it to comprise two hydrophobic integral membrane proteins, CeuB (35.5 kDa) and CeuC (34.8 kDa), which may form the cytoplasmic membrane permease, an ATP-binding protein, CeuD (28.8 kDa),and a periplasmic substrate-binding protein, CeuE (34.5 kDa). In vivolabelling studies using [H-3]palmitate demonstrated that CeuE, the periplasmic binding protein, is expressed as a lipoprotein in C. coli, which is unusual for a Cram-negative PET system. Mutants of C. coli, defective in components of the transport mechanism, were severely impaired in the ability to utilize enterochelin as an iron source suggestingthat this siderophore is a substrate for the transport system, This is the first molecular characterization of a PET system in Campylobacter species.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 23:34:10