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Titolo:
DIRECT-SEQUENCE DATA FROM HETEROGENEOUS CREATINE-KINASE (43 KDA) BY HIGH-RESOLUTION TANDEM MASS-SPECTROMETRY
Autore:
WOOD TD; CHEN LH; KELLEHER NL; LITTLE DP; KENYON GL; MCLAFFERTY FW;
Indirizzi:
UNIV CALIF SAN FRANCISCO,DEPT PHARMACEUT CHEM SAN FRANCISCO CA 94143 UNIV CALIF SAN FRANCISCO,DEPT PHARMACEUT CHEM SAN FRANCISCO CA 94143 CORNELL UNIV,DEPT CHEM,BAKER LAB ITHACA NY 14853
Titolo Testata:
Biochemistry
fascicolo: 50, volume: 34, anno: 1995,
pagine: 16251 - 16254
SICI:
0006-2960(1995)34:50<16251:DDFHC(>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIPLY CHARGED IONS; ELECTROSPRAY IONIZATION; LARGE MOLECULES; MUSCLE; PRINCIPLES; CLONING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
T.D. Wood et al., "DIRECT-SEQUENCE DATA FROM HETEROGENEOUS CREATINE-KINASE (43 KDA) BY HIGH-RESOLUTION TANDEM MASS-SPECTROMETRY", Biochemistry, 34(50), 1995, pp. 16251-16254

Abstract

Isoelectric focusing separation of recombinant rabbit muscle creatinekinase (CK) and its (282)Cys --> (282)Ser mutant shows the presence of three and two isoforms, respectively, that exhibit equivalent enzymatic activity. Electrospray ionization coupled with Fourier-transform mass spectrometry (10(5) resolving power) of both CKs indicates that their major components are within +/-2 Da of the M(r) value predicted from the cDNA sequences of these mixtures. Dissociation of (M + nH)(n+) gives no evidence that the components of either CK are isomers; the masses of the 51 fragment ions correlate completely (+/-1 Dal with the values predicted from the cDNA sequence and confirm the identities of 21 of the 380 amino acids and the (282)Cys --> (282)Ser replacement in the mutant. The results are consistent with one or two steps of post-translational amidation/deamidation (NH2 --> OH, 16 Da --> 17 Da), eachof which would produce only a 1 Da difference in M(r), with the fragment masses indicating that at least one modification occurs between residues 212 and 282.

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Documento generato il 09/07/20 alle ore 13:50:52