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Titolo:
MICROTUBULE REARRANGEMENT DURING IN-VITRO MATURATION OF PIG OOCYTES -EFFECT OF CYCLOHEXIMIDE
Autore:
ROZINEK J; PETR J; GROCHOLOVA R; JILEK F;
Indirizzi:
RES INST ANIM PROD,DEPT REPROD BIOL & MOLEC GENET,10 UHRINEVES PRAGUECZECH REPUBLIC INST CARE MOTHER & CHILD,IVF LAB PRAGUE CZECH REPUBLIC PRAGUE AGR UNIV,DEPT VET SCI PRAGUE CZECH REPUBLIC
Titolo Testata:
Reproduction, nutrition, development
fascicolo: 6, volume: 35, anno: 1995,
pagine: 685 - 694
SICI:
0926-5287(1995)35:6<685:MRDIMO>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
GERMINAL VESICLE BREAKDOWN; MOUSE OOCYTES; MEIOTIC MATURATION; PROTEIN-SYNTHESIS; NUCLEAR MATURATION; MAMMALIAN OOCYTE; BOVINE OOCYTES; XENOPUS EGGS; INVITRO; TAXOL;
Keywords:
PIG OOCYTE; MEIOTIC MATURATION; MICROTUBULE; CYCLOHEXIMIDE; TAXOL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
J. Rozinek et al., "MICROTUBULE REARRANGEMENT DURING IN-VITRO MATURATION OF PIG OOCYTES -EFFECT OF CYCLOHEXIMIDE", Reproduction, nutrition, development, 35(6), 1995, pp. 685-694

Abstract

In freshly isolated fully grown pig oocytes at the germinal vesicle (GV) stage, the cytoplasmic microtubules are arranged in a meshwork. This microtubule arrangement is also maintained during the initial phases of meiotic maturation in vitro. A perinuclear array of microtubules is formed immediately before germinal vesicle breakdown (GVBD). Short-term treatment of oocytes with taxol when oocytes are at metaphase I stage induced formation of cytoplasmic asters. The oocyte cytoplasm is unable to respond to the taxol treatment at the earlier stages of meiotic maturation. In oocytes cultured with the proteosynthesis inhibitorcycloheximide, meiotic maturation is blocked. Condensation of chromatin occurs but the nuclear envelope is preserved and the microtubule arrangement is unchanged. A perinuclear array of microtubules does not appear and oocyte cytoplasm does not respond to shortterm taxol treatment by the formation of cytoplasmic asters. We can conclude that the microtubule rearrangement and the acquisition of competence for tubulin assembly are blocked by cycloheximide and are thus dependent on de novo proteosynthesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 09:59:05