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Titolo:
EVIDENCE FOR THE INVOLVEMENT OF A NUCLEAR NF-KAPPA-B INHIBITOR IN GLOBAL DOWN-REGULATION OF THE MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-I ENHANCER IN ADENOVIRUS TYPE 12-TRANSFORMED CELLS
Autore:
LIU XH; GE RW; RICCIARDI RP;
Indirizzi:
UNIV PENN,SCH DENT MED,DEPT MICROBIOL,4010 LOCUST ST PHILADELPHIA PA 19104 UNIV PENN,SCH DENT MED,DEPT MICROBIOL PHILADELPHIA PA 19104
Titolo Testata:
Molecular and cellular biology
fascicolo: 1, volume: 16, anno: 1996,
pagine: 398 - 404
SICI:
0270-7306(1996)16:1<398:EFTIOA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSFORMED RAT-CELLS; TRANSCRIPTION FACTOR; BINDING-ACTIVITY; GENE-EXPRESSION; MHC; ACTIVATION; SUBUNIT; DNA; REL; PRECURSOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
X.H. Liu et al., "EVIDENCE FOR THE INVOLVEMENT OF A NUCLEAR NF-KAPPA-B INHIBITOR IN GLOBAL DOWN-REGULATION OF THE MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-I ENHANCER IN ADENOVIRUS TYPE 12-TRANSFORMED CELLS", Molecular and cellular biology, 16(1), 1996, pp. 398-404

Abstract

Diminished expression of major histocompatibility complex class I antigens on the surface of adenovirus type 12 (Ad12)-transformed cells contributes to their high tumorigenic potential by enabling them to escape immune recognition by cytotoxic T lymphocytes. This low class I antigen expression is due to a block in class I transcription, which is mediated by Ad12 E1A. Genetic analysis has shown that the class I enhancer is the target for transcriptional down-regulation. In this study, we show that the ability of the R1 element of the class I enhancer to stimulate transcription is greatly reduced in Ad12-transformed cells. The loss of functional activity by the R1 element was attributed to loss of binding by the NF-kappa B p50-p65 heterodimer, NF-kappa B binding appears to be blocked within the nucleus rather than at the level ofnuclear translocation, Significantly, NF-kappa B binding activity could be recovered from the nuclear extracts of Ad12-transformed cells following detergent treatment, suggesting that the block is mediated through a nuclear inhibitor present in the Ad12-transformed cells. These results, taken together with the fact that the R2 element of the classI enhancer exhibits strong binding to the transcriptional repressor COUP-TF, suggest that the class I enhancer is globally down-regulated in Ad12-transformed cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 09:31:00