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Titolo:
DOPAMINERGIC INHIBITION OF CATECHOLAMINE SECRETION FROM CHROMAFFIN CELLS - EVIDENCE THAT INHIBITION IS MEDIATED BY D4 AND D5 DOPAMINE-RECEPTORS
Autore:
DAHMER MK; SENOGLES SE;
Indirizzi:
UNIV TENNESSEE,COLL MED,DEPT BIOCHEM,858 MADISON AVE,SUITE G01 MEMPHIS TN 38163
Titolo Testata:
Journal of neurochemistry
fascicolo: 1, volume: 66, anno: 1996,
pagine: 222 - 232
SICI:
0022-3042(1996)66:1<222:DIOCSF>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADRENAL-MEDULLA; MOLECULAR-CLONING; PROLACTIN-RELEASE; ADENYLATE-CYCLASE; CALCIUM CHANNELS; LACTOTROPH CELLS; RAT; EXPRESSION; GENE; NEUROLEPTICS;
Keywords:
ADRENAL GLAND; CA2+ UPTAKE; PERTUSSIS TOXIN; ADENYLYL CYCLASE; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
M.K. Dahmer e S.E. Senogles, "DOPAMINERGIC INHIBITION OF CATECHOLAMINE SECRETION FROM CHROMAFFIN CELLS - EVIDENCE THAT INHIBITION IS MEDIATED BY D4 AND D5 DOPAMINE-RECEPTORS", Journal of neurochemistry, 66(1), 1996, pp. 222-232

Abstract

Previous studies have suggested that activation of D2-like dopamine receptors inhibits catecholamine secretion from adrenal chromaffin cells. The purpose of this study was to determine whether the activation of D1-like receptors on chromaffin cells affects either catecholamine release from the cells or the inhibition of secretion by D2-like dopamine receptors, Both D1- and D2-selective agonists inhibited secretion elicited by dimethylphenyl-piperazinium (DMPP), veratridine, and high K levels, The D1-selective agonists allyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine (Cl-APB) and SKF-38393 inhibited DMPP-stimulated catecholamine secretion in a concentration-dependent manner; 50% inhibition was obtained with similar to 10 mu M Cl-APB and similar to 100 mu M SKF-38393, Of the D2-selective agonists, bromocriptine was a more potent inhibitor of DMPP-stimulated catecholamine release than was quinpirole. The inhibition of secretion caused by Cl-APB or SKF-38393 was additive with the inhibition caused by bromocriptine. Pertussis toxin treatment (50 ng/ml, 18 h) attenuated the inhibitory effect of D2-selective, but not D1-selective, dopamine agonists. In addition, forskolin-stimulated adenylyl cyclase activity was inhibited by D2-selective, but not D1-selective, agonists, Neither D1- nor D2-selective agonists stimulated adenylyl cyclase activity in the cells, although cyclase activity was stimulated by forskolin, carbachol, and vasoactive intestinal peptide, DMPP-stimulated Ca2+ uptake was inhibited by both D1- and D2-selective dopamine agonists. PCR analysis was used to determine which of the dopamine receptor subtypes within the D1-like and D2-like subfamilies was responsible for the observed inhibition. PCR analysis indicated that mRNA for only D4 and D5 dopamine receptor subtypes was present in chromaffin cells. These combined data suggest that D1- and D2-selective agonists inhibit Ca2+ uptake and catecholamine secretion by activating D4 and D5 dopamine receptors on chromaffin cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 17:41:48