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Titolo:
CHARACTERIZATION OF THE GLUTAMATE RECEPTORS MEDIATING RELEASE OF SOMATOSTATIN FROM CULTURED HIPPOCAMPAL-NEURONS
Autore:
FONTANA G; DEBERNARDI R; FERRO F; GEMIGNANI A; RAITERI M;
Indirizzi:
IST FARMACOL & FARMACOGNOSIA,VIALE CEMBRANO 4 I-16148 GENOA ITALY IST FARMACOL & FARMACOGNOSIA I-16148 GENOA ITALY
Titolo Testata:
Journal of neurochemistry
fascicolo: 1, volume: 66, anno: 1996,
pagine: 161 - 168
SICI:
0022-3042(1996)66:1<161:COTGRM>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
TEMPORAL-LOBE EPILEPSY; EXCITATORY AMINO-ACIDS; 2 DISTINCT MECHANISMS; KAINIC ACID; STRIATAL NEURONS; CORTICAL-NEURONS; INDUCED SEIZURES; CEREBRAL-CORTEX; RAT HIPPOCAMPUS; EVOKED RELEASE;
Keywords:
SOMATOSTATIN RELEASE; GLUTAMATE RECEPTORS; GLUTAMIC ACID; NMDA RECEPTORS; DL-ALPHA-AMINO-3-HYDROXY-5-METHYL-4-ISOXAZOLE PROPIONATE RECEPTORS; CULTURED HIPPOCAMPAL NEURONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
G. Fontana et al., "CHARACTERIZATION OF THE GLUTAMATE RECEPTORS MEDIATING RELEASE OF SOMATOSTATIN FROM CULTURED HIPPOCAMPAL-NEURONS", Journal of neurochemistry, 66(1), 1996, pp. 161-168

Abstract

L-Glutamate, NMDA, DL-alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and kainate (KA) increased the release of somatostatin-like immunoreactivity (SRIF-LI) from primary cultures of rat hippocampal neurons. In Mg2+-containing medium, the maximal effects (reached atsimilar to 100 mu M) amounted to 737% (KA), 722% (glutamate), 488% (NMDA), and 374% (AMPA); the apparent affinities were 22 mu M (AMPA), 39mu M (glutamate), 41 mu M (KA), and 70 mu M (NMDA). The metabotropic receptor agonist trans-1-aminocyclopentane-1,3-dicarboxylate did not affect SRIF-LI release. The release evoked by glutamate (100 mu M) was abolished by 10 mu M dizocilpine (MK-801) plus 30 mu M 1-aminophenyl-4-methyl-7,8- methylenedioxy-5H-2,3-benzodiazepine (GYKI 52466). Moreover, the maximal effect of glutamate was mimicked by a mixture of NMDA AMPA. The release elicited by NMDA was sensitive to MK-801 but insensitive to GYKI 52466. The AMPA- and KA-evoked releases were blocked by6,7-dinitroquinoxaline-2,3-dione (DNQX) or by GYKI 52466 but were insensitive to MK-801. The release of SRIF-LI elicited by all four agonists was Ca2+ dependent, whereas only the NMDA-evoked release was prevented by tetrodotoxin. Removal of Mg2+ caused increase of basal SRIF-LI release, an effect abolished by MK-801. Thus, glutamate can stimulate somatostatin release through ionotropic NMDA and AMPA/KA receptors. Receptors of the KA type (AMPA insensitive) or metabotropic receptors appear not to be involved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 23:56:43