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Titolo:
EXPRESSION OF HUMAN METALLOTHIONEIN-III CONFERS PROTECTION AGAINST SERUM-FREE EXPOSURE OF STABLY TRANSFECTED CHINESE-HAMSTER OVARY CHO-K1 CELLS
Autore:
AMOUREUX MC; WURCH T; PAUWELS PJ;
Indirizzi:
CTR RECH PIERRE FABRE,CELLULAR & MOLEC NEUROBIOL LAB,17 AVE JEAN MOULIN F-81106 CASTRES FRANCE CTR RECH PIERRE FABRE,CELLULAR & MOLEC NEUROBIOL LAB F-81106 CASTRES FRANCE
Titolo Testata:
Neuroscience letters
fascicolo: 1, volume: 201, anno: 1995,
pagine: 61 - 64
SICI:
0304-3940(1995)201:1<61:EOHMCP>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-INHIBITORY FACTOR; ALZHEIMERS-DISEASE; MOLECULAR-CLONING; CYTO-TOXICITY; FACTOR CDNA; BRAIN; GLUTATHIONE; INDUCTION; SURVIVAL; NEURONS;
Keywords:
METALLOTHIONEIN-III (CLONED, HUMAN); CHINESE HAMSTER OVARY (CHO-K1) CELL LINE; PERMANENT TRANSFECTION; REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION; CELL SURVIVAL; SERUM STARVATION; GROWTH FACTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
M.C. Amoureux et al., "EXPRESSION OF HUMAN METALLOTHIONEIN-III CONFERS PROTECTION AGAINST SERUM-FREE EXPOSURE OF STABLY TRANSFECTED CHINESE-HAMSTER OVARY CHO-K1 CELLS", Neuroscience letters, 201(1), 1995, pp. 61-64

Abstract

The cloned human metallothionein (MT)-III coding region was permanently transfected in Chinese hamster ovary (CHO-K1) cells in order to investigate the growth regulatory effects of this brain-specific protein. Reverse transcription-polymerase chain reaction (RT-PCR) experiments demonstrated stable expression of MT-III mRNA in CHO-K1/MT-III cells. Whereas in the presence of serum no differences were observed between the cell growth of both CHO-K1/MT-III and CHO-K1/pcDNA3-plasmid transfected cells, cell survival was attenuated differently in serum-free medium. CHO-K1/MT-III cells were more resistant (40 +/- 8%) to serum deprivation than pcDNAS-plasmid transfected cells. Recovery of cell growth for both cell lines was obtained by supplementing serum (0.25%), although with a different growth rate; half-maximal 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT)-conversion was observedbetween 5.1-5.7 and 4.2-4.5 days for CHO-K1/pcDNA3 and CHO-K1/MT-III cells, respectively. These results suggest a protective role for cloned human MT-III, besides its reported inhibitory and stimulatory effects on cell survival.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:37:56