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Titolo:
CLONING, NUCLEOTIDE-SEQUENCE AND CHARACTERIZATION OF A GENE ENCODING SUPEROXIDE-DISMUTASE FROM CAMPYLOBACTER-JEJUNI AND CAMPYLOBACTER-COLI
Autore:
PURDY D; PARK SF;
Indirizzi:
INST FOOD RES,READING LAB,EARLEY GATE,WHITEKNIGHTS RD READING RG6 2EFBERKS ENGLAND INST FOOD RES,READING LAB READING RG6 2EF BERKS ENGLAND
Titolo Testata:
Microbiology
, volume: 140, anno: 1994,
parte:, 5
pagine: 1203 - 1208
SICI:
1350-0872(1994)140:<1203:CNACOA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; DNA;
Keywords:
CAMPYLOBACTER JEJUNI; CAMPYLOBACTER COLI; SUPEROXIDE DISMUTASE; CLONING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
D. Purdy e S.F. Park, "CLONING, NUCLEOTIDE-SEQUENCE AND CHARACTERIZATION OF A GENE ENCODING SUPEROXIDE-DISMUTASE FROM CAMPYLOBACTER-JEJUNI AND CAMPYLOBACTER-COLI", Microbiology, 140, 1994, pp. 1203-1208

Abstract

Genes encoding superoxide dismutase (SOD: EC 1.15.1.1) were cloned from Campylobacter jejuni NCTC 11351 and Campylobacter coli UA585 by heterologous complementation of a SOD-deficient Escherichia coli mutant. Deletion analysis of the cloned C. jejuni DNA assigned the sod gene toa 1.2 kb insert and this contained an open reading frame of 660 bp. The deduced gene product of 220 amino acids was 71% identical to the E.coli iron-containing SOD and 60% identical to the E. coli manganese-containing SOD. The recombinant SOD was expressed at high levels in E. coli and protected a sodA sodB double mutant from the toxic effects ofmethyl viologen. Nucleotide sequence analysis of the corresponding gene from C. coil showed it to be 92% identical to that from C. jejuni.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 15:54:37