Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
6-ALPHA-METHYLHYDROCORTISONE 1-EN-DEHYDROGENATION BY ARTHROBACTER-GLOBIFORMIS-193 CELLS
Autore:
ARINBASAROVA AY; FOKINA VV; KARPOV AV; MEDENTSEV AG; KOSHCHEYENKO KA;
Indirizzi:
RUSSIAN ACAD SCI,INST MICROORGANISMS BIOCHEM & PHYSIOL PUSHCHINO 142292 RUSSIA
Titolo Testata:
Biochemistry
fascicolo: 10, volume: 60, anno: 1995,
pagine: 1285 - 1291
SICI:
0006-2979(1995)60:10<1285:61BA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Keywords:
3-KETOSTEROID-1-EN-DEHYDROGENASE; ARTHROBACTER GLOBIFORMIS 193; 6-ALPHA-METHYLHYDROCORTISONE; 1-EN-DEHYDROGENATION; MICROBIOLOGICAL TRANSFORMATION; SUBSTRATE INHIBITION; MATHEMATICAL MODEL; PARAMETER IDENTIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
11
Recensione:
Indirizzi per estratti:
Citazione:
A.Y. Arinbasarova et al., "6-ALPHA-METHYLHYDROCORTISONE 1-EN-DEHYDROGENATION BY ARTHROBACTER-GLOBIFORMIS-193 CELLS", Biochemistry, 60(10), 1995, pp. 1285-1291

Abstract

Some peculiarities of 6 alpha-methylhydrocortisone 1-en-dehydrogenation by Arthrobacter globiformis 193 cells have been studied. The introduction of an additional methyl group into the hydrocortisone molecule changes the biochemical characteristics of the microbiological 1-en-dehydrogenation. The process as a whole follows substrate inhibition kinetics rather than Michaelis-Menten kinetics. A mathematical model for 6 alpha-methylhydrocortisone 1-en-dehydrogenation by whole cells whichdescribes multiple uncompetitive inhibition by the substrate is proposed. All kinetic parameters of this reaction have been estimated. The relationship between the substrate 1-en-dehydrogenation and oxygen consumption has been studied. The inhibiting effect of 6 alpha-methylhydrocortisone appears at the level of electron transfer along the respiratory chain. At low substrate concentrations (up to 1.06 mM), when the inhibitory effect is virtually absent, the reaction rate is determinedby enzyme-substrate interaction rather than by respiratory chain activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 14:30:52