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Titolo:
HORMONAL AND NUTRITIONAL CONTROL OF THE FATTY-ACID SYNTHASE PROMOTER IN TRANSGENIC MICE
Autore:
SONCINI M; YET SF; MOON YH; CHUN JY; SUL HS;
Indirizzi:
UNIV CALIF BERKELEY,DEPT NUTR SCI BERKELEY CA 94720 UNIV CALIF BERKELEY,DEPT NUTR SCI BERKELEY CA 94720
Titolo Testata:
The Journal of biological chemistry
fascicolo: 51, volume: 270, anno: 1995,
pagine: 30339 - 30343
SICI:
0021-9258(1995)270:51<30339:HANCOT>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-TRANSCRIPTION; CARBOHYDRATE REGULATION; INSULIN; EXPRESSION; LIVER; DIFFERENTIATION; SYNTHETASE; GLUCOSE; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
M. Soncini et al., "HORMONAL AND NUTRITIONAL CONTROL OF THE FATTY-ACID SYNTHASE PROMOTER IN TRANSGENIC MICE", The Journal of biological chemistry, 270(51), 1995, pp. 30339-30343

Abstract

To study the molecular basis of tissue-specific and hormonally regulated expression of the fatty acid synthase (FAS) gene in vivo, we generated lines of transgenic mice carrying 2.1 kilobases of the 5'-flanking region (-2100 to +67) of the rat FAS gene fused to a chloramphenicolacetyltransferase (CAT) reporter gene. This reporter gene construct was strongly expressed in tissues that normally express high levels of FAS mRNA, which include liver and white adipose tissues, In contrast, CAT reporter activity was not detected in appreciable levels in lung, heart, kidney, and muscle tissues, which do not normally show significant levels of FAS activity, The relative levels of the CAT mRNA drivenby the rat FAS promoter in various tissues of the transgenic animals approximated those of the endogenous mouse FAS mRNA, We also examined the hormonal and nutritional regulation of the FAS(2.1)-CAT reporter gene in transgenic mice, CAT activity was increased in both liver and white adipose tissue when fasted animals were refed a high carbohydrate, fat-free diet, These changes in CAT activity and CAT mRNA levels occurred in parallel to the changes in endogenous mouse FAS mRNA levels, On the other hand, fasting/refeeding did not change CAT activity appreciably in other tissues, such as muscle and brown adipose tissue, Administration of dibutyryl cAMP at the start of refeeding prevented an increase in CAT activity in liver, However, the cAMP effect was tissue-specific as cAMP treatment did not bring about change in CAT activity in adipose tissue, Next, to examine the effect of insulin, we made the transgenic mice insulin-deficient by streptozotocin treatment, Insulintreatment of the streptozotocin-diabetic mice increased both the CAT activity and CAT mRNA levels driven by the rat FAS promoter in liver and white adipose tissue, These changes in CAT expression by insulin paralleled those in endogenous FAS mRNA levels, Administration of glucocorticoids increased CAT activity in all tissues examined: liver, whiteand brown adipose tissues, lung, heart, and spleen, Overall, the first 2.1 kilobases of the 5'-flanking region of the rat FAS gene appear to contain sequence elements necessary to confer tissue-specific and hormonally regulated expression characteristic of the endogenous FAS gene.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 05:23:45