Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
RET ACTIVATION BY GERMLINE MEN2A AND MEN2B MUTATIONS
Autore:
BORRELLO MG; SMITH DP; PASINI B; BONGARZONE I; GRECO A; LORENZO MJ; ARIGHI E; MIRANDA C; ENG C; ALBERTI L; BOCCIARDI R; MONDELLINI P; SCOPSI L; ROMEO G; PONDER BAJ; PIEROTTI MA;
Indirizzi:
IST NAZL TUMORI,DIV EXPTL ONCOL A,VIA VENEZIAN 1 I-20133 MILAN ITALY IST NAZL TUMORI,DIV EXPTL ONCOL A I-20133 MILAN ITALY UNIV CAMBRIDGE,ADDENBROOKES HOSP,CRC,HUMAN CANC GENET RES GRP CAMBRIDGE CB2 2QQ ENGLAND HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DEPT MED BOSTON MA 02115 IST GIANNINA GASLINI,GENET MOLEC LAB I-16147 GENOA ITALY IST NAZL TUMORI,DIV ANAT PATHOL & CYTOL I-20133 MILAN ITALY
Titolo Testata:
Oncogene
fascicolo: 11, volume: 11, anno: 1995,
pagine: 2419 - 2427
SICI:
0950-9232(1995)11:11<2419:RABGMA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIPLE ENDOCRINE NEOPLASIA; NEUROBLASTOMA CELL-LINES; PROTO-ONCOGENE; TYROSINE KINASE; PROTOONCOGENE; EXPRESSION; DIFFERENTIATION; CARCINOMAS; SEQUENCE; GENE;
Keywords:
RET; RECEPTOR TYROSINE KINASE; MULTIPLE ENDOCRINE NEOPLASIA TYPE 2; INHERITED CANCER SYNDROME; ONCOGENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
M.G. Borrello et al., "RET ACTIVATION BY GERMLINE MEN2A AND MEN2B MUTATIONS", Oncogene, 11(11), 1995, pp. 2419-2427

Abstract

The RET proto-oncogene encodes a receptor tyrosine kinase (TK), It has been shown that distinct germline mutations in the RET proto-oncogene are associated with the dominantly inherited cancer syndromes multiple endocrine neoplasia type 2A and 2B (MEN 2A and MEN 2B) and familialmedullary thyroid carcinoma (FMTC) as well as Hirschsprung disease (HSCR), a congenital disorder characterised by absent enteric innervation, In this study, we have transfected NIH3T3 and PC12 phaeochromocytoma cells with MEN2A (Cys634-->Arg) and MEN2B (Met918-->Thr) RET constructs, Both caused transformation of the NIH3T3 cells and differentiation of PC12 cells, The Bet (MEN2A) and Bet (MEN2B) proteins were constitutively phosphorylated on tyrosine, and their in vitro kinase activitywas significantly higher than that of the wild type protein, The MTC cell line TT carries a Cys634-->Trp MEN2A mutation, and we have shown by immunoelectronmicroscopy that Ret is clustered on the cell surface in a manner reminiscent of ligand-induced aggregation of cell surface receptors, RET is activated, as RET/PTC oncogene, by somatic rearrangements which link the TK domain to a constitutive dimerization interface in papillary thyroid carcinomas, We have compared the biological andbiochemical activity of the TK domains of the wild type and MEN 2B Ret in the context of the RET/PTC, The results show that the MEN2B mutation significantly increases the TK domain enzymatic activity suggesting that dimerization may be still necessary for MEN 2B Bet to express its full activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 04:58:01