Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CYTOSKELETAL CONTROL OF MYOGENESIS - A DESMIN NULL MUTATION BLOCKS THE MYOGENIC PATHWAY DURING EMBRYONIC STEM-CELL DIFFERENTIATION
Autore:
WEITZER G; MILNER DJ; KIM JU; BRADLEY A; CAPETANAKI Y;
Indirizzi:
BAYLOR COLL MED,DEPT CELL BIOL,1 BAYLOR PLAZA HOUSTON TX 77030 BAYLOR COLL MED,DEPT CELL BIOL HOUSTON TX 77030 BAYLOR COLL MED,HOWARD HUGHES MED INST HOUSTON TX 77030 BAYLOR COLL MED,INST GENET MOLEC HOUSTON TX 77030
Titolo Testata:
Developmental biology
fascicolo: 2, volume: 172, anno: 1995,
pagine: 422 - 439
SICI:
0012-1606(1995)172:2<422:CCOM-A>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERMEDIATE FILAMENT ORGANIZATION; GENE-EXPRESSION; TRANSGENIC MICE; NUCLEAR-ENVELOPE; SKELETAL-MUSCLE; PLASMA-MEMBRANE; AVIAN ERYTHROCYTES; MOUSE EMBRYO; VIMENTIN; INVITRO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
98
Recensione:
Indirizzi per estratti:
Citazione:
G. Weitzer et al., "CYTOSKELETAL CONTROL OF MYOGENESIS - A DESMIN NULL MUTATION BLOCKS THE MYOGENIC PATHWAY DURING EMBRYONIC STEM-CELL DIFFERENTIATION", Developmental biology, 172(2), 1995, pp. 422-439

Abstract

A differentiating system based on embryonic stem (ES) cell-derived embryoid bodies (EBs) which recapitulates the in vivo cardiac, skeletal,and smooth muscle myogenesis of mouse embryos was developed and used to investigate the effects of the disruption of the desmin gene on muscle cell differentiation. Wild-type, heterozygous, and homozygous celllines with the mutated desmin allele were evaluated. Skeletal myogenesis Tvas totally inhibited in desmin null mutant EBs, as manifested bythe absence of myotube formation, contractility, and myoD, myogenin, myf5, and myosin heavy chain expression. Smooth muscle formation was also completely blocked in the absence of desmin, On the other hand, there were no obvious effects on cardiomyocyte differentiation in these desmin null mutant EBs. However, reduced desmin expression in EBs heterozygous for the desmin mutation leads to partial inhibition of cardiac muscle formation. These data suggest that in contrast to early cardiocyte differentiation, desmin is indispensable for skeletal and smoothmuscle formation. (C) 1995 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 20:04:22