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Titolo:
METABOLISM OF THE HERBICIDE CHLORTOLURON BY HUMAN CYTOCHROME-P450 3A4
Autore:
MEHMOOD Z; KELLY DE; KELLY SL;
Indirizzi:
UNIV SHEFFIELD,KREBS INST BIOMOLEC RES,DEPT MOLEC BIOL & BIOTECHNOL SHEFFIELD S10 2UH S YORKSHIRE ENGLAND UNIV SHEFFIELD,KREBS INST BIOMOLEC RES,DEPT MOLEC BIOL & BIOTECHNOL SHEFFIELD S10 2UH S YORKSHIRE ENGLAND
Titolo Testata:
Chemosphere
fascicolo: 11-12, volume: 31, anno: 1995,
pagine: 4515 - 4529
SICI:
0045-6535(1995)31:11-12<4515:MOTHCB>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-SUSPENSION CULTURES; CHLOROTOLURON;
Keywords:
CYTOCHROME P450 3A4; YEAST EXPRESSION; CHLORTOLURON; BIOTRANSFORMATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
Z. Mehmood et al., "METABOLISM OF THE HERBICIDE CHLORTOLURON BY HUMAN CYTOCHROME-P450 3A4", Chemosphere, 31(11-12), 1995, pp. 4515-4529

Abstract

Studies were carried out to investigate the metabolism of herbicide chlortoluron in the microsomal fractions and whole cells of Saccharomyces cerevisiae expressing human cytochrome P450 3A4. Both whole cells and microsomal fractions of yeast expressing human cytochrome P450 3A4 exhibited a typical dithionite-reduced, CO-difference absorbance spectrum with maximum absorbance at 448 nm. Chlortoluron produced a type I binding spectrum with cytochrome P450 3A4 with a K-s value of 200 mu M. Chlortoluron was metabolised into four metabolites; hydroxylated-N-monodemethylated, hydroxylated ring methylated, N-didemethylated and N-monodemethylated products. Chlortoluron metabolism was absolutely dependent on NADPH and no metabolism was observed in control transformants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/02/20 alle ore 13:58:05