Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A MICROSCALE ELECTROSPRAY INTERFACE FOR ONLINE, CAPILLARY LIQUID-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY OF COMPLEX PEPTIDE MIXTURES
Autore:
DAVIS MT; STAHL DC; HEFTA SA; LEE TD;
Indirizzi:
CITY HOPE NATL MED CTR,BECKMAN RES INST DUARTE CA 91010 CITY HOPE NATL MED CTR,BECKMAN RES INST DUARTE CA 91010
Titolo Testata:
Analytical chemistry
fascicolo: 24, volume: 67, anno: 1995,
pagine: 4549 - 4556
SICI:
0003-2700(1995)67:24<4549:AMEIFO>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
M.T. Davis et al., "A MICROSCALE ELECTROSPRAY INTERFACE FOR ONLINE, CAPILLARY LIQUID-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY OF COMPLEX PEPTIDE MIXTURES", Analytical chemistry, 67(24), 1995, pp. 4549-4556

Abstract

A microcapillary liquid chromatography (HPLC) system designed for thegradient elution of peptide and protein samples at now rates < 1 mu L/min has been coupled to a triple-sector quadrupole mass spectrometer via a simple sheathless electrospray interface (microspray). The microspray interface used a name-drawn, uncoated, fused silica needle with tip outer diameters in the range of 15-20 mu m and an opening less than 5 mu m in diameter. Online sample filtration to prevent clogging of the drawn needle was accomplished by using a hydrophilic PVDF membranefilter integrated into the needle assembly. The spray potential (0.5-1 kV) was applied directly to the sample stream through the capillary union, Stable electrospray conditions were obtained over the full range of the gradient (0-90% acetonitrile in water) and was generally independent of flow rate. Both off-line and online analyses of proteins and peptide digest mixtures were performed at sample levels less than 10fmol. HPLC parameters could be optimized for either rapid LC/MS analysis or enhanced performance in LC/MS/MS experiments by modulation of the eluting peak widths. Additionally, flow could be greatly reduced asselected components pass through the interface to prolong the time available to collect mass spectral data. The reduced spectral backgroundand peak width manipulation facilitated the acquisition of peptide product ion spectra (MS/MS) using real-time, automated instrument control procedures.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/10/20 alle ore 12:36:08