Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
SPATIALLY-ADDRESSABLE IMMOBILIZATION OF MACROMOLECULES ON SOLID SUPPORTS
Autore:
SANDBERG SA; BARRETT RW; PIRRUNG M; LU AL; KIANGSOONTRA B; HOLMES CP;
Indirizzi:
AFFYMAX RES INST,3410 CENT EXPRESSWAY SANTA CLARA CA 95051 AFFYMAX RES INST SANTA CLARA CA 95051 DUKE UNIV,DEPT CHEM,PAUL M GROSS CHEM LAB DURHAM NC 27708
Titolo Testata:
Journal of the American Chemical Society
fascicolo: 49, volume: 117, anno: 1995,
pagine: 12050 - 12057
SICI:
0002-7863(1995)117:49<12050:SIOMOS>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTECTING GROUPS; DERIVATIVES; BIOTIN; PHOTOCHEMISTRY; STREPTAVIDIN; RECOGNITION; MONOLAYERS; SURFACES; BINDING; ANALOGS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
S.A. Sandberg et al., "SPATIALLY-ADDRESSABLE IMMOBILIZATION OF MACROMOLECULES ON SOLID SUPPORTS", Journal of the American Chemical Society, 117(49), 1995, pp. 12050-12057

Abstract

A method is described for immobilization of receptors, antibodies, orother macromolecules at precise locations on solid substrates. We have combined photolithographic techniques with the use of a ''caged'' biotin analogue that has been covalently linked to the substrate surface. Exposure to near UV light through a photolithographic mask yields biotin sites for streptavidin binding. Biotinylated macromolecules are then immobilized via a biotin-streptavidin-biotin bridge. Molecules maybe attached at selected locations by carrying out repeated rounds of exposure, streptavidin binding, and application of the biotinylated reagent. We have demonstrated the immobilization of fluorescein-streptavidin molecules in 500 mu m x 500 mu m sites, and the localization of two biotinylated antibodies at different sites on a planar substrate surface. We anticipate that the technique will prove useful in drug screening, diagnostics, and biosensor applications.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/11/20 alle ore 20:07:49