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Titolo:
MOLECULAR AND IMMUNOHISTOCHEMICAL STUDY OF CLASS-I GROWTH-FACTOR RECEPTORS IN SQUAMOUS-CELL LUNG CARCINOMAS
Autore:
GORGOULIS V; SFIKAKIS PP; KARAMERIS A; PAPASTAMATIOU H; TRIGIDOU R; VESLEMES M; SPANDIDOS DA; SFIKAKIS P; JORDANOGLOU J;
Indirizzi:
53 ANTAIOU STR GR-11146 ATHENS GREECE GEN ARMY HOSP 401,DEPT PATHOL ATHENS GREECE UNIV ATHENS,SOTIRIA HOSP,SCH MED,DEPT PULM DIS ATHENS GREECE SOTIRIA HOSP,DEPT PATHOL ATHENS GREECE NATL HELLEN RES FDN,INST BIOL RES & BIOTECHNOL ATHENS GREECE UNIV ATHENS,LAIKON GEN HOSP,SCH MED,DEPT PROPEDEUT MED 1,RES IMMUNOL LAB ATHENS GREECE
Titolo Testata:
Pathology research and practice
fascicolo: 10, volume: 191, anno: 1995,
pagine: 973 - 981
SICI:
0344-0338(1995)191:10<973:MAISOC>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; C-ERBB-3 PROTEIN; ARCHIVAL TISSUE; GENE-PRODUCT; EXPRESSION; CANCER; ADENOCARCINOMAS; EGF; ONCOGENES; SURVIVAL;
Keywords:
C-ERBB-1; C-ERBB-2; C-ERBB-3; SQUAMOUS CELL LUNG CARCINOMA; GENE AMPLIFICATION; IMMUNOHISTOCHEMISTRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
V. Gorgoulis et al., "MOLECULAR AND IMMUNOHISTOCHEMICAL STUDY OF CLASS-I GROWTH-FACTOR RECEPTORS IN SQUAMOUS-CELL LUNG CARCINOMAS", Pathology research and practice, 191(10), 1995, pp. 973-981

Abstract

The class I growth factor receptor family includes epidermal growth factor receptor, i.e. c-erbB-1, c-erbB-2 and c-erbB-3 molecules. These receptors have a significant sequence homology and play an important role in cell growth and differentiation. To further investigate their implication in squamous cell lung carcinomas (SqCLCs), we studied the protein expression by immunohistochemistry and examined for possible gene amplification by a novel semi-quantitative differential polymerase chain reaction (DPCR) technique. Expression of c-erbB-1, c-erbB-2 and c-erbB-3 was present in 65%, 28% and 10% respectively, of 40 SqCLCs cases. Seven of the 11 cases that expressed c-erbB-2, was well as all 4 c-erbB-3 expressing cases, also stained with the anti-c-erbB-1 mAb. Expression of c-erbB-1, but not c-erbB-2 or c-erbB-3, correlated with the grade of tumor differentiation (100%, 64% and 36% positive cases of well, moderately and poorly differentiated cases respectively, p < 0.003). In addition, c-erbB-1 expression correlated with the presence of regional lymph node metastases within the moderately differentiated group. The c-erbB-1 gene was amplified in 11/40 (28%) cases, all of which overexpressed c-erbB-1 protein, while c-erbB-2 gene amplification was detected in only one case. There was no c-erbB-3 gene amplification in any of the 40 SqCLCs cases. These findings suggest that c-erbB-1, c-erbB-2 and c-erbB-3 receptors do not have a common role and are of different physiological importance, at least at the stage of clinically overt tumor in human SqCLCs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 06:07:49