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Titolo:
CENTRAL AND PERIPHERAL CHOLECYSTOKININ RECEPTORS IN CHICKENS DIFFER FROM THOSE IN MAMMALS
Autore:
RODRIGUEZSINOVAS A; FERNANDEZ AG; GONALONS E;
Indirizzi:
AUTONOMOUS UNIV BARCELONA,FAC VET,DEPT CELL BIOL & PHYSIOL E-08193 BARCELONA SPAIN
Titolo Testata:
Regulatory peptides
fascicolo: 1, volume: 60, anno: 1995,
pagine: 47 - 54
SICI:
0167-0115(1995)60:1<47:CAPCRI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
FUNCTIONAL EXPRESSION; MOLECULAR-CLONING; HUMAN BRAIN; CHROMOSOMAL LOCALIZATION; CCK-A; GASTRIN; PANCREAS; ANTAGONISTS; MEMBRANES; PEPTIDE;
Keywords:
CHOLECYSTOKININ (CCK) RECEPTOR; L-364,718; L-365,260; CHICKEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
A. Rodriguezsinovas et al., "CENTRAL AND PERIPHERAL CHOLECYSTOKININ RECEPTORS IN CHICKENS DIFFER FROM THOSE IN MAMMALS", Regulatory peptides, 60(1), 1995, pp. 47-54

Abstract

Specific binding for the radioligand [H-3]CCK-8s has been identified in chicken brain, hypothalamus, pancreas, gallbladder and caecum membranes. This binding was found to be of high affinity, low capacity and saturable, suggesting the presence of specific CCK receptors in these tissues. Scatchard analysis indicated the existence of a single binding site for each tissue. Dissociation constant (k(d)) values were 0.63 /- 0.18, 0.73 +/- 0.13, 0.85 +/- 0.12, 1.47 +/- 0.21 and 0.96 nM for brain, hypothalamus, pancreas, caecum and gallbladder, respectively. Binding densities (B-max) were higher for brain, pancreas and caecum (32.60 +/- 10.70, 30.33 +/- 2.40 and 35.83 +/- 5.10 fmol/mg protein, respectively) than for the other two tissues (9.75 +/- 1.90 and 6.31 fmol/mg protein for hypothalamus and gallbladder, respectively). As in mammals, CCK-4 shows high affinity for CCK receptors located in chicken brain and hypothalamus, and very low affinity for those located in peripheral structures. L-364,718 (a CCK-A antagonist) showed a relative selectivity and a high affinity for those receptors located in central tissues, whereas L-365,260 (a CCK-B antagonist) is almost inactive in all studied tissues. These results give support for the existence of atleast two distinct CCK receptors in birds and that these receptors are relatively different from those described in mammals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:30:14