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Titolo:
ANTIVIRAL ACTIVITY OF AN OLIGO(NUCLEOSIDE METHYLPHOSPHONATE) THAT TARGETS HSV-1 IMMEDIATE-EARLY PRE-MESSENGER-RNA-4,5 IS AUGMENTED BY COTREATMENT WITH REPLICATION-DEFECTIVE ADENOVIRUS
Autore:
KULKA M; AURELIAN L;
Indirizzi:
UNIV MARYLAND,SCH MED,DEPT PHARMACOL & EXPTL THERAPEUT,VIROL IMMUNOL LABS,10 S PINE ST BALTIMORE MD 21201 UNIV MARYLAND,SCH MED,DEPT PHARMACOL & EXPTL THERAPEUT,VIROL IMMUNOL LABS BALTIMORE MD 21201 JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DEPT BIOCHEM BALTIMORE MD 21205
Titolo Testata:
Antisense research and development
fascicolo: 4, volume: 5, anno: 1995,
pagine: 243 - 249
SICI:
1050-5261(1995)5:4<243:AAOAOM>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL GROWTH-FACTOR; MEDIATED GENE DELIVERY; EARLY MESSENGER RNA-4; CELLULAR UPTAKE; OLIGODEOXYRIBONUCLEOSIDE METHYLPHOSPHONATES; ANTISENSE OLIGONUCLEOTIDES; SPLICE JUNCTION; PENTON BASE; COMPLEMENTARY; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
M. Kulka e L. Aurelian, "ANTIVIRAL ACTIVITY OF AN OLIGO(NUCLEOSIDE METHYLPHOSPHONATE) THAT TARGETS HSV-1 IMMEDIATE-EARLY PRE-MESSENGER-RNA-4,5 IS AUGMENTED BY COTREATMENT WITH REPLICATION-DEFECTIVE ADENOVIRUS", Antisense research and development, 5(4), 1995, pp. 243-249

Abstract

Replication-defective adenovirus p259A caused a 400-fold increase in the sequence-specific antiherpetic activity of oligo(nucleoside methylphosphonate) (ONMP) IE4,5SA. Herpes simplex virus type 1 (HSV-1) growth was not inhibited in cells exposed to p259A in the absence of IE4,5SA or in cells cotreated with IE4,5SA and heated (10 minutes, 90 degrees C) p259A virus, Fluorescent microscopy of Vero cells treated with BODIPY-conjugated IE4,5SA revealed intracellular localization within endocytic-like vesicles with minimal cytoplasmic and intranuclear distribution. Diffuse staining over the entire cell was observed in cell cotreated with the BODIPY-conjugated IE4,5SA and p259A virus, This effect was not observed in cells cotreated with the BODIPY-conjugated ONMP and heated p259A virus. We interpret these findings to indicate that p259A augments IE4,5SA antiherpetic activity presumably via its ability to increase ONMP uptake and release from endocytic-like vesicles.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 11:55:23