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Titolo:
COMPLETE NUCLEOTIDE-SEQUENCE, EXPRESSION, AND CHROMOSOMAL LOCALIZATION OF HUMAN MIXED-LINEAGE KINASE-2
Autore:
DOROW DS; DEVEREUX L; TU GF; PRICE G; NICHOLL JK; SUTHERLAND GR; SIMPSON RJ;
Indirizzi:
PETER MACCALLUM CANC INST,DIV RES MELBOURNE VIC 3000 AUSTRALIA LUDWIG INST CANC RES,JOINT PROT STRUCT LAB,MELBOURNE BRANCH MELBOURNEVIC AUSTRALIA WALTER & ELIZA HALL INST MED RES PARKVILLE VIC AUSTRALIA WOMENS & CHILDRENS HOSP,DEPT CYTOGENET & MOLEC GENET,CTR GENET MED ADELAIDE SA AUSTRALIA
Titolo Testata:
European journal of biochemistry
fascicolo: 2, volume: 234, anno: 1995,
pagine: 492 - 500
SICI:
0014-2956(1995)234:2<492:CNEACL>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR TYROSINE KINASES; POLYMERASE CHAIN-REACTION; LIGAND-BINDING SITE; SH3 DOMAINS; PROTEIN-KINASE; LEUCINE ZIPPER; CRYSTAL-STRUCTURE; FAMILY; IDENTIFICATION; PHOSPHORYLATION;
Keywords:
PROTEIN KINASE; MIXED-LINEAGE KINASE; LEUCINE ZIPPER; SH3 DOMAIN; DNA SEQUENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
D.S. Dorow et al., "COMPLETE NUCLEOTIDE-SEQUENCE, EXPRESSION, AND CHROMOSOMAL LOCALIZATION OF HUMAN MIXED-LINEAGE KINASE-2", European journal of biochemistry, 234(2), 1995, pp. 492-500

Abstract

Protein kinases play pivotal roles in the control of many cellular processes, In a search for protein kinases expressed in human epithelialtumour cells, we discovered two members of a novel protein kinase family [Dorow, D. S., Devereux, L., Dietzsch, E. & de Kretser, T. A. (1993) Eur. J. Bioch. 213, 701-710]. Due to the unique mixture of structural domains within their amino acid sequences, we named the family mixed-lineage kinases (MLK). We initially isolated clones encoding partialcDNAs of MLK1 and 2 from a human colonic cDNA library. The MLK2 cDNA was subsequently used to screen a human brain cDNA library and we havenow cloned and sequenced a 3454-bp cDNA encoding the full-length MLK2protein. The predicted MLK2 polypeptide has 954 amino acids and contains a src homology 3 (SH3) domain, a kinase catalytic domain, a doubleleucine zipper and basic domain, and a large C-terminal domain. The 22-amino-acid N-terminal region has four glutamic acid residues immediately following the initiator methionine. Beginning at amino acid 23, the 55-amino-ncid SH3 domain contains a 5-amino-acid insert in a position corresponding to inserts of 6 and 15 residues in the SH3 domains ofn-src and the phosphatidylinositol 3'-kinase. Adjacent to the SH3 domain is a kinase catalytic domain with conserved motifs associated withboth serine/threonine and tyrosine specificity. Beginning nine residues C-terminal to the catalytic domain, there are two leucine/isoleucine zippers separated by a 13-amino-acid spacer sequence and followed bya stretch of basic residues, The polybasic sequence contains a motif that is similar to nuclear localisation signals from several proteins. The C-terminal domain is composed of 491 amino acids of which 17% areserine or threonine and 16% are proline, This domain also has a biased ratio of basic to acidic amino acids with a calculated pl of 9.38. When used as a probe to examine mRNA expression in human tissues, a MLK2 cDNA hybridised to a species of 3.8 kb that was expressed at highestlevels in RNA from brain and skeletal muscle, The 3454-bp cDNA was also used for fluorescence in situ hybridisation to localise the MLK2 gene to human chromosome 19 q13.2.

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Documento generato il 21/09/20 alle ore 18:30:29