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Titolo:
IN-VITRO PROLACTIN-RELEASE FROM PITUITARIES OF OVARIECTOMIZED, ESTRADIOL-TREATED HOLTZMAN RATS - A DIRECT COMPARISON OF DISPERSED CELLS ANDTISSUE EXPLANTS
Autore:
HU LM; LAWSON DM;
Indirizzi:
WAYNE STATE UNIV,SCH MED,DEPT PHYSIOL,540 E CANFIELD AVE DETROIT MI 48201 WAYNE STATE UNIV,SCH MED,DEPT PHYSIOL DETROIT MI 48201
Titolo Testata:
Life sciences
fascicolo: 3, volume: 58, anno: 1995,
pagine: 229 - 237
SICI:
0024-3205(1995)58:3<229:IPFPOO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTERIOR-PITUITARY; GLAND; ANGIOTENSIN; IMMUNOREACTIVITY; COMMUNICATION; SECRETION; ESTROGEN;
Keywords:
PROLACTIN; PITUITARY EXPLANTS; DISPERSED CELLS; DOPAMINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
L.M. Hu e D.M. Lawson, "IN-VITRO PROLACTIN-RELEASE FROM PITUITARIES OF OVARIECTOMIZED, ESTRADIOL-TREATED HOLTZMAN RATS - A DIRECT COMPARISON OF DISPERSED CELLS ANDTISSUE EXPLANTS", Life sciences, 58(3), 1995, pp. 229-237

Abstract

In this study we compared prolactin (PRL) release from pituitary explants with that from dispersed cells from two regions of anterior pituitaries obtained from ovariectomized rats treated for 7 days with estradiol. Pituitaries were cut horizontally into dorsal (or inner) and ventral (or outer) zones. One half of each of these regions was enzymatically dispersed to cells; the other half was bisected and examined as tissue explants. The dispersed cells and explants were perifused in parallel over a 2 hour period with dopamine (1 mu M) present for the first hour and absent during the second hour. Prolactin released into the perifusion medium was measured by radioimmunoassay. Tissue explants secreted more total prolactin than did cells in the presence or absence of dopamine and the pattern of release was sustained compared to the more transient release observed in dispersed cells. However, the response to dopamine withdrawal was not different between cells and explantswhen expressed as a percent change from respective controls. The results suggest that organization and architecture of the rat anterior pituitary may be important factors for overall prolactin production, but in terms of relative responsiveness to dopamine and its withdrawal, dispersed cells and tissue explants appear equivalent.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 11:25:09