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Titolo:
INTRINSIC ISOTACHOPHORETIC PRECONCENTRATION IN CAPILLARY GEL-ELECTROPHORESIS OF DNA RESTRICTION FRAGMENTS
Autore:
VANDERSCHANS MJ; BECKERS JL; MOLLING MC; EVERAERTS FM;
Indirizzi:
EINDHOVEN UNIV TECHNOL,INSTRUMENTAL ANAL LAB,POB 513 5600 MB EINDHOVEN NETHERLANDS
Titolo Testata:
Journal of chromatography
fascicolo: 1-2, volume: 717, anno: 1995,
pagine: 139 - 147
Fonte:
ISI
Lingua:
ENG
Soggetto:
ZONE ELECTROPHORESIS; SEPARATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
M.J. Vanderschans et al., "INTRINSIC ISOTACHOPHORETIC PRECONCENTRATION IN CAPILLARY GEL-ELECTROPHORESIS OF DNA RESTRICTION FRAGMENTS", Journal of chromatography, 717(1-2), 1995, pp. 139-147

Abstract

Reproducible migration times are required for accurate base-pair assignment of polymerase chain reaction (PCR) products in capillary gel electrophoresis (CGE). However, migration times are influenced by injection plug length and ionic strength of the sample. In this paper we introduce a new isotachophoresis (ITP)-CGE system where the transition from ITP to CGE is achieved by the mobility shift of DNA from free solution (ITP) to sieving gel buffer (CGE). With intrinsic isotachophoreticpreconcentration in capillary gel electrophoresis (IICGE) large volume injections (up to 700 nl) are possible with accurate migration timesin CGE independent of injection plug length or sample ionic strength.

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Documento generato il 26/09/20 alle ore 17:13:53