Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
HUMAN ATRIAL-NATRIURETIC-PEPTIDE GENE DELIVERY REDUCES BLOOD-PRESSUREIN HYPERTENSIVE RATS
Autore:
LIN KF; CHAO J; CHAO L;
Indirizzi:
MED UNIV S CAROLINA,DEPT BIOCHEM & MOLEC BIOL,171 ASHLEY AVE CHARLESTON SC 29425 MED UNIV S CAROLINA,DEPT BIOCHEM & MOLEC BIOL CHARLESTON SC 29425
Titolo Testata:
Hypertension
fascicolo: 6, volume: 26, anno: 1995,
parte:, 1
pagine: 847 - 853
SICI:
0194-911X(1995)26:6<847:HAGDRB>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPSTEIN-BARR VIRUS; LONG-TERM; SYSTEMIC DELIVERY; CHRONIC INFUSION; MUSCLE INVIVO; HORMONE; CELLS; ANF; ATRIOPEPTINS; ELECTROLYTE;
Keywords:
ATRIAL NATRIURETIC FACTOR; RATS, INBRED SHR; SOMATIC GENE THERAPY; BLOOD PRESSURE; HYPERTENSION;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
K.F. Lin et al., "HUMAN ATRIAL-NATRIURETIC-PEPTIDE GENE DELIVERY REDUCES BLOOD-PRESSUREIN HYPERTENSIVE RATS", Hypertension, 26(6), 1995, pp. 847-853

Abstract

Chronic infusion of atrial natriuretic peptide (ANP) has been shown to cause natriuresis, diuresis, and hypotension in rats and humans. We explored the effect of a continuous supply of ANP by somatic ANP delivery on genetically hypertensive rats. A DNA construct containing the human ANP gene fused to the Rous sarcoma virus 3'-long terminal repeat (RSV-LTR) was injected intravenously into spontaneously hypertensive rats (SHR) through the tail vein. Expression of human ANP in SHR was identified in the heart, lung, and kidney by radioimmunoassay and reverse transcription-polymerase chain reaction followed by Southern blot analysis. A single injection of naked ANP plasmid DNA (12.3 kb) caused asignificant reduction of systemic blood pressure in young SHR (4 weeks old), and the effect continued for 7 weeks. The differences were significant at 1 to 2 weeks (n=6, P<.05) and 3 to 6 weeks after injection(n=6, P<.01) A maximal blood pressure reduction of 21 mm Hg in young SHR was observed 5 weeks after injection with ANP DNA (159.4 +/- 3.02 mm Hg, mean+/-SEM, n=6) compared with SHR injected with vector DNA alone (180.2+/-3.02 mm Hg, mean+/-SEM; n=6 P<.01). Somatic gene delivery of human ANP DNA had no effect on the blood pressure of adult SHR (12 weeks old). After ANP gene delivery, there were significant increases in urinary volume and urinary potassium output (n=6, P<.05) but not inbody weight, heart rate, water intake, urinary sodium output, urinarycreatinine, and urinary protein. Antibodies to human ANP or plasmid ANP DNA were not detected in rat sera. These results indicate that somatic delivery of the human ANP gene induces a sustained reduction of systemic blood pressure in young hypertensive rats and raise the feasibility of using ANP gene therapy for the treatment of human hypertension.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 23:59:27