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Titolo:
OCCURRENCE OF HEMOGLOBIN IN THE NITROGEN-FIXING ROOT-NODULES OF ALNUS-GLUTINOSA
Autore:
SUHARJO UKJ; TJEPKEMA JD;
Indirizzi:
UNIV MAINE,DEPT PLANT BIOL & PATHOL ORONO ME 04469 UNIV MAINE,DEPT PLANT BIOL & PATHOL ORONO ME 04469
Titolo Testata:
Physiologia Plantarum
fascicolo: 2, volume: 95, anno: 1995,
pagine: 247 - 252
SICI:
0031-9317(1995)95:2<247:OOHITN>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
PARASPONIA; CASUARINA; MYOGLOBIN; PROTEINS; PLANTS;
Keywords:
ACTINORHIZAL NODULES; ALDER; ALNUS GLUTINOSA; FRANKIA; HEMOGLOBIN; NITROGEN FIXATION; OXYGEN DIFFUSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
U.K.J. Suharjo e J.D. Tjepkema, "OCCURRENCE OF HEMOGLOBIN IN THE NITROGEN-FIXING ROOT-NODULES OF ALNUS-GLUTINOSA", Physiologia Plantarum, 95(2), 1995, pp. 247-252

Abstract

A true hemoglobin (Hb) was shown to be present in the root nodules ofAlnus glutinosa L. After purification by gel filtration and ion exchange, the Hb formed a stable complex with oxygen. This oxygen complex could then be converted to carboxyhemoglobin by treatment with CO. Optical absorption spectra typical of Hb were observed. The molecular weight was estimated to be 15 100 by gel filtration, and 18 300 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The Hb was largelyinsoluble when the initial homogenization was done in the absence of a detergent. Under these conditions much of the Hb appears to be associated with clusters of Frankia, the nitrogen-fixing actinomycete that infects plant cells within the nodules. The exact localization of the Hb in vivo is uncertain. The relatively low average concentration of Hb in Alnus nodules suggests that it is either confined to a relativelysmall fraction of total nodule volume, or has a function other than facilitation of O-2 transport.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 20:46:07