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Titolo:
BINDING OF HEMOGLOBIN BY PORPHYROMONAS-GINGIVALIS
Autore:
AMANO A; KUBONIWA M; KATAOKA K; TAZAKI K; INOSHITA E; NAGATA H; TAMAGAWA H; SHIZUKUISHI S;
Indirizzi:
OSAKA UNIV,FAC DENT,DEPT PREVENT DENT,1-8 YAMADAOKA SUITA OSAKA 565 JAPAN
Titolo Testata:
FEMS microbiology letters
fascicolo: 1, volume: 134, anno: 1995,
pagine: 63 - 67
SICI:
0378-1097(1995)134:1<63:BOHBP>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMIN-BINDING; PROTEINS; RADIOACTIVITY; LIMITATION; W50;
Keywords:
PORPHYROMONAS GINGIVALIS; HEMOGLOBIN; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
A. Amano et al., "BINDING OF HEMOGLOBIN BY PORPHYROMONAS-GINGIVALIS", FEMS microbiology letters, 134(1), 1995, pp. 63-67

Abstract

In this study, we investigated whether Porphyromonas gingivalis can bind hemoglobin as an initial step in the acquisition of heme from hemoglobin. The binding of human hemoglobin by P. gingivalis cells was determined using [H-3]hemoglobin. Hemoglobin binding occurred rapidly, reversibly and specifically. A Scatchard analysis of the binding data generated a linear plot, indicating a single population of binding proteins. The apparent K-d was 1.0+/-0.19X10(-6) M and there were 3.2+/-0.76X10(4) binding sites per cell. Hemoglobin binding was inhibited by unlabeled human hemoglobin but not by hemin and protoporphyrin IX. The binding was only partially inhibited by human serum albumin, transferrin, lactoferrin, catalase and cytochrome c. These results suggest that the ligand recognized by the binding protein may not be the heme moiety. The binding of hemoglobin considerably increased when the organismswere grown under hemin-limited conditions. Hemoglobin bound to outer membrane proteins extracted from P. gingivalis cells on a dot blot binding assay and binding ability was lost after heating bacterial proteins. These results suggest that P. gingivalis cells interact with humanhemoglobin through specific binding sites on their surfaces as a preliminary step in iron acquisition.

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Documento generato il 29/11/20 alle ore 00:49:11