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Titolo:
NEURONAL DIFFERENTIATION MODIFIES THE EFFECT OF ETHANOL EXPOSURE ON VOLTAGE-DEPENDENT CALCIUM CHANNELS IN NG-108-15 CELLS
Autore:
BERGAMASCHI S; BATTAINI F; TRABUCCHI M; PARENTI M; LOPEZ CM; GOVONI S;
Indirizzi:
UNIV MILAN,INST PHARMACOL SCI,VIA BALZARETTI 9 I-20133 MILAN ITALY UNIV MILAN,INST PHARMACOL SCI I-20133 MILAN ITALY UNIV ROMA TOR VERGATA,DEPT EXPTL MED & BIOCHEM SCI ROME ITALY UNIV MILAN,SCH MED,DEPT PHARMACOL MILAN ITALY
Titolo Testata:
Alcohol
fascicolo: 6, volume: 12, anno: 1995,
pagine: 497 - 503
SICI:
0741-8329(1995)12:6<497:NDMTEO>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
OMEGA-CONOTOXIN; PC12 CELLS; RAT-BRAIN; CATECHOLAMINE RELEASE; PEPTIDE RELEASE; MODULATION; TERMINALS; CURRENTS; BINDING; SYNAPTOSOMES;
Keywords:
ETHANOL; CALCIUM CHANNELS; NG 108-15; CELL CULTURE; NEURONAL DIFFERENTIATION; OMEGA-CONOTOXIN; PN200-110;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
S. Bergamaschi et al., "NEURONAL DIFFERENTIATION MODIFIES THE EFFECT OF ETHANOL EXPOSURE ON VOLTAGE-DEPENDENT CALCIUM CHANNELS IN NG-108-15 CELLS", Alcohol, 12(6), 1995, pp. 497-503

Abstract

The effect of prolonged (72 h) ethanol (200 mM) exposure on the labeling of L-type (using tritiated PN 200-110) and N-type (using iodinatedomega-conotoxin) voltage-dependent calcium channels was investigated in cultured NG 108-15 cells. In undifferentiated cells ethanol produced an 80% increase in PN 200-110 B-max and no changes in omega-conotoxin binding. Differentiation had a profound effect on the response of cells to ethanol, which in differentiated neuron-like cells decreased omega-conotoxin binding (-53.5%) leaving PN 200-110 labeling of L-type channels unaffected. The effect was time dependent and reversible upon ethanol withdrawal. The decreased omega-conotoxin binding was accompanied by a reduced ability of omega-conotoxin to inhibit K+-stimulated calcium uptake. The results demonstrate that in cultured NG 108-15 cells ethanol differentially affects DHP and omega-conotoxin-sensitive, voltage-dependent calcium channels and that the effect is also modulatedby differentiation of the cell to a neuronal phenotype.

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Documento generato il 28/11/20 alle ore 21:35:16