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Titolo:
COLLAGEN-LIKE COMPLEMENT COMPONENT C1Q IS A MEMBRANE-PROTEIN OF HUMANMONOCYTE-DERIVED MACROPHAGES THAT MEDIATES ENDOCYTOSIS
Autore:
KAUL M; LOOS M;
Indirizzi:
INST MED MICROBIOL & HYG,HOCHHAUS AM AUGUSTPL D-55101 MAINZ GERMANY INST MED MICROBIOL & HYG D-55101 MAINZ GERMANY
Titolo Testata:
The Journal of immunology
fascicolo: 12, volume: 155, anno: 1995,
pagine: 5795 - 5802
SICI:
0022-1767(1995)155:12<5795:CCCCIA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
1ST COMPONENT; FUNCTIONAL-PROPERTIES; SUBCOMPONENT C1Q; SIGNAL SEQUENCE; BINDING-SITE; RECEPTOR; CELLS; INTERNALIZATION; IDENTIFICATION; PHAGOCYTOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
M. Kaul e M. Loos, "COLLAGEN-LIKE COMPLEMENT COMPONENT C1Q IS A MEMBRANE-PROTEIN OF HUMANMONOCYTE-DERIVED MACROPHAGES THAT MEDIATES ENDOCYTOSIS", The Journal of immunology, 155(12), 1995, pp. 5795-5802

Abstract

The collagen-like Clq molecule, a subcomponent of the first componentof complement, C1, is synthesized by macrophages (M phi). Previously,we have demonstrated that Clq is a membrane protein of guinea pig peritoneal macrophages (M phi). To extend this observation as a general biologic characteristic of M phi, we investigated human (hu) monocyte-derived M phi. Interestingly, surface labeling with the biotin derivative idyl-6-(biotinamido)-hexanoate(biotinamido)-hexano of M phi, freshly isolated monocytes, lymphocytes, granulocytes, and myelomonocytic U937 cells revealed that Clq occurs only on the surface of M phi and noton the surface of the other cells types. Therefore, Clq appears to bea marker for differentiation into M phi. FITC-labeled, fixed Staphylococcus aureus coupled to membrane C1q via a monoclonal alpha-hu-Clq Abwere used to demonstrate that membrane Clq is capable of mediating phagocytosis. Various detergents (Nonidet P-40, digitonin, lubrol, and Triton X-114) were used to solubilize membrane Clq. Membrane Clq of hu M phi is tightly bound to or located in the intact membrane, since treatment of cells with acidic buffers (''acid strip'') failed to remove Clq from the cell surface. However, repeated freezing and thawing of cells and washing of segregated membranes with buffer containing 1 M KCl and 3 M urea brought about a marked release of membrane C1q.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 05:31:22