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Titolo:
MOLECULAR CHARACTERIZATION OF RECOMBINANT GREEN FLUORESCENT PROTEIN BY FLUORESCENCE CORRELATION MICROSCOPY
Autore:
TERRY BR; MATTHEWS EK; HASELOFF J;
Indirizzi:
UNIV CAMBRIDGE,DEPT PHARMACOL,TENNIS COURT RD CAMBRIDGE CB2 1QJ ENGLAND UNIV CAMBRIDGE,DEPT PHARMACOL CAMBRIDGE CB2 1QJ ENGLAND BIOIMAGE DK-2860 SOBORG DENMARK MRC,MOLEC BIOL LAB CAMBRIDGE CB2 2QH ENGLAND
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 1, volume: 217, anno: 1995,
pagine: 21 - 27
SICI:
0006-291X(1995)217:1<21:MCORGF>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORRELATION SPECTROSCOPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
B.R. Terry et al., "MOLECULAR CHARACTERIZATION OF RECOMBINANT GREEN FLUORESCENT PROTEIN BY FLUORESCENCE CORRELATION MICROSCOPY", Biochemical and biophysical research communications, 217(1), 1995, pp. 21-27

Abstract

The cDNA for the green fluorescent protein (GFP) of Aequorea victoriahas been expressed in transformed cells of Saccharomyces cerevisiae and the recombinant GFP isolated. Protonation and deprotonation of the cloned and purified GFP produced major effects on its spectral absorption characteristics with an increase in pH enhancing the fluorescence emission of the GFP more than twofold. Finally, molecular characterisation of GFP by fluorescence correlation microscopy in a minimal targetvolume of 1 fL yielded a translational diffusion coefficient (D-T) of8.7x10(-7) cm(2).sec(-1), equivalent to a Stokes radius of 2.82nm fora monodisperse globular protein of 27kDa. (C) Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:41:42