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Titolo:
PRODUCTION OF HYDROXYECTOINE - HIGH CELL-DENSITY CULTIVATION AND OSMOTIC DOWNSHOCK OF MARINOCOCCUS STRAIN M52
Autore:
FRINGS E; SAUER T; GALINSKI EA;
Indirizzi:
UNIV BONN,INST MIKROBIOL & BIOTECHNOL,MECKENHEIMER ALLEE 168 D-53115 BONN GERMANY UNIV BONN,INST MIKROBIOL & BIOTECHNOL D-53115 BONN GERMANY
Titolo Testata:
Journal of biotechnology
fascicolo: 1, volume: 43, anno: 1995,
pagine: 53 - 61
SICI:
0168-1656(1995)43:1<53:POH-HC>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPATIBLE SOLUTES; HALOPHILIC EUBACTERIA; ESCHERICHIA-COLI; ECTOTHIORHODOSPIRA-HALOCHLORIS; GLYCINE BETAINE; AMINO-ACID; TETRAHYDROPYRIMIDINES; STRESS; PROTECTION; BACTERIUM;
Keywords:
MARINOCOCCUS SP; COMPATIBLE SOLUTE; ENZYME PROTECTANT; TETRAHYDROPYRIMIDINE; HYDROXYECTOINE; FED-BATCH CULTIVATION; OSMOTIC DOWNSHOCK;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
E. Frings et al., "PRODUCTION OF HYDROXYECTOINE - HIGH CELL-DENSITY CULTIVATION AND OSMOTIC DOWNSHOCK OF MARINOCOCCUS STRAIN M52", Journal of biotechnology, 43(1), 1995, pp. 53-61

Abstract

This study reports the mass-cultivation of a Gram-positive halophilicmicroorganism for the biotechnological production of the compatible solute and enzyme protectant hydroxyectoine. A high cell-density cultivation of the hydroxyectoine producer strain M52 (Marinococcus sp.) wasperformed using an exponential fed-batch strategy with medium exchange. Grown on a complex glycine betaine-free medium containing 10% (w/v)salt, 56 g cell dry weight per 1 were produced as compared to only 6 g l(-1) in a batch process. The desired product hydroxyectoine accounted for 13.5% of the cellular dry weight. As strain M52 tolerates extreme changes of the surrounding osmolarity, the extraction and purification of hydroxyectoine could be simplified by washing the dense cultureof M52 prior to cell harvest using demineralized water (osmotic downshock). Hydroxyectoine was then easily extracted from the washed and 'desalted' cell material by Soxhlet extraction with methanol.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 03:21:22