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Titolo:
LIQUID-CHROMATOGRAPHIC DETERMINATION OF CLOZAPINE AND N-DESMETHYLCLOZAPINE IN HUMAN SERUM USING SOLID-PHASE EXTRACTION
Autore:
GUPTA RN;
Indirizzi:
ST JOSEPHS HOSP,DEPT LAB MED,50 CHARLTON AVE E HAMILTON ON L8N 4A6 CANADA
Titolo Testata:
Journal of chromatography B. Biomedical applications
fascicolo: 2, volume: 673, anno: 1995,
pagine: 311 - 315
Fonte:
ISI
Lingua:
ENG
Soggetto:
ULTRAVIOLET DETECTION; HUMAN-PLASMA; NORCLOZAPINE;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
R.N. Gupta, "LIQUID-CHROMATOGRAPHIC DETERMINATION OF CLOZAPINE AND N-DESMETHYLCLOZAPINE IN HUMAN SERUM USING SOLID-PHASE EXTRACTION", Journal of chromatography B. Biomedical applications, 673(2), 1995, pp. 311-315

Abstract

A 0.5-ml aliquot of a serum sample, after the addition of a 50-mu l aliquot of a 5 mu g/ml solution of amoxapine as the internal standard, is vortex-mixed with 0.5 mi of acetonitrile and centrifuged. The supernatant is applied to a 1-ml BondElut C-18 silica extraction column-conditioned with subsequent washings with 1 M HCl, methanol and water. After passing the sample at a slow rate, the column is washed twice withwater and once with acetonitrile. The desired compounds are then eluted with a 0.25-ml aliquot of 35% perchloric acid-methanol (1:100, v/v). A 15-mu l aliquot of the eluate is injected onto a 150 x 4.6 mm I.D.column packed with 5-mu m C-8 silica particles and eluted at ambient temperature with a mobile phase of 0.1% tetramethylammonium perchlorate-acetonitrile (73:27, v/v) adjusted to pH 4.2 with 10% perchloric acid. The peaks are detected with an absorbance detector at 245 nm.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/20 alle ore 20:26:53