Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF A 3RD ISOFORM OF THE HUMAN CALCITONIN RECEPTOR AND PARTIAL CHARACTERIZATION OF THE CALCITONIN RECEPTOR GENE
Autore:
ALBRANDT K; BRADY EMG; MOORE CX; MULL E; SIERZEGA ME; BEAUMONT K;
Indirizzi:
AMYLIN PHARMACEUT INC,DEPT PHARMACOL,9373 TOWNE CTR DR,SUITE 250 SAN DIEGO CA 92121
Titolo Testata:
Endocrinology
fascicolo: 12, volume: 136, anno: 1995,
pagine: 5377 - 5384
SICI:
0013-7227(1995)136:12<5377:MAFEOA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASOACTIVE-INTESTINAL-PEPTIDE; HORMONE-RELATED PEPTIDE; POLYPEPTIDE RECEPTOR; PARATHYROID-HORMONE; RAT-BRAIN; AFFINITY; CELLS; PACAP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
55
Recensione:
Indirizzi per estratti:
Citazione:
K. Albrandt et al., "MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF A 3RD ISOFORM OF THE HUMAN CALCITONIN RECEPTOR AND PARTIAL CHARACTERIZATION OF THE CALCITONIN RECEPTOR GENE", Endocrinology, 136(12), 1995, pp. 5377-5384

Abstract

We have cloned and expressed two isoforms of the human calcitonin (hCT) receptor. Primers designed from the published sequence of a CT receptor cloned from an ovarian small cell carcinoma line were used for the polymerase chain reaction amplification of related products from human breast carcinoma MCF-7 cells. Two complementary DNAs were isolated. One clone lacks a 16-amino acid insert in the first intracellular loop and is virtually identical to the receptor recently cloned from the T47D human breast carcinoma cell line. The second clone is another splice variant lacking both the 16-amino acid insert in the first intracellular domain as well as the first 47 amino acids of the amino-terminus extracellular domain. COS-7 cells transfected with either receptor isoform bound [I-125]salmon CT with high affinity and responded to hCT with increases in cAMP. Tissue distribution studies revealed the truncated extracellular domain 1 isoform transcripts in human skeletal muscle, kidney, brain, and lung. Analysis of a hCT receptor genomic clone demonstrated an exon/intron organization similar to that of the porcine CT receptor gene, except for a distinct exon coding for the alternatively spliced insert in the first intracellular domain.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 15:11:38