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Titolo:
A NICOTINIC ACETYLCHOLINE-RECEPTOR REGULATING CELL-ADHESION AND MOTILITY IS EXPRESSED IN HUMAN KERATINOCYTES
Autore:
GRANDO SA; HORTON RM; PEREIRA EFR; DIETHELMOKITA BM; GEORGE PM; ALBUQUERQUE EX; CONTIFINE BM;
Indirizzi:
UNIV MINNESOTA,SCH MED,DEPT DERMATOL,POB 98 UMHC MINNEAPOLIS MN 55455 UNIV MINNESOTA,SCH MED,DEPT PHARMACOL MINNEAPOLIS MN 55455 UNIV MINNESOTA,COLL BIOL SCI,DEPT BIOCHEM ST PAUL MN 55108 UNIV MARYLAND,SCH MED,DEPT PHARMACOL & EXPTL THERAPEUT BALTIMORE MD 21201 FED UNIV RIO DE JANEIRO,INST BIOFIS CARLOS CHAGAS FILHO,MOLEC PHARMACOL LAB BR-21945 RIO JANEIRO BRAZIL
Titolo Testata:
Journal of investigative dermatology
fascicolo: 6, volume: 105, anno: 1995,
pagine: 774 - 781
SICI:
0022-202X(1995)105:6<774:ANARCA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CULTURED HUMAN KERATINOCYTES; MONOCLONAL-ANTIBODIES; CALCIUM PERMEABILITY; SUBUNIT; BUNGAROTOXIN; CHANNELS; NEURONS; SKIN; LOCALIZATION; OUTGROWTH;
Keywords:
PATCH CLAMP; RADIOLIGAND BINDING; IMMUNOFLUORESCENCE; POLYMERASE CHAIN REACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
S.A. Grando et al., "A NICOTINIC ACETYLCHOLINE-RECEPTOR REGULATING CELL-ADHESION AND MOTILITY IS EXPRESSED IN HUMAN KERATINOCYTES", Journal of investigative dermatology, 105(6), 1995, pp. 774-781

Abstract

Acetylcholine is synthesized and released by human epidermal keratinocytes and modulates the adhesion and motility of these cells. To understand the molecular basis of the effects of acetylcholine on keratinocytes, we investigated the presence, pharmacology, structure, and function of nicotinic acetylcholine receptors in human epidermal keratinocytes. Patch-clamp studies indicated that keratinocytes express acetylcholine receptors with ion gating and pharmacologic properties similar to those observed so far only in neurons, and containing the alpha(3) subunit. Specific binding of the receptor-specific ligand I-125-kappa-bungarotoxin revealed approximately 5500 binding sites per cell on undifferentiated keratinocytes in cell cultures and approximately 35,400 binding sites per cell on mature keratinocytes freshly isolated from human neonatal foreskins. Antibody binding and polymerase chain reactionexperiments demonstrated the presence of alpha(3), beta(2), and beta(4) nicotinic receptor subunits. Binding of subunit-specific antibodiesindicated that nicotinic receptors were associated with the suprabasal keratinocytes in epidermis and localized to the cell membranes of differentiated keratinocytes in cell cultures. Acetylcholine and the nicotinic agonist nicotine increased cell-substrate and cell-cell adherence of cultured keratinocytes and stimulated their lateral migration. The specific antagonists K-bungarotoxin and mecamylamine caused cell detachment and abolished migration. Thus, a nicotinic receptor expressedin keratinocytes may mediate acetylcholine control of keratinocyte adhesion and motility.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 09:41:08