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Titolo:
METHANOL DOES NOT SPECIFICALLY INHIBIT ENDOGENOUS BETA-GLUCURONIDASE (GUS) ACTIVITY
Autore:
WILKINSON JE; TWELL D; LINDSEY K;
Indirizzi:
UNIV LEICESTER,DEPT BOT LEICESTER LE1 7RH ENGLAND UNIV LEICESTER,DEPT BOT LEICESTER LE1 7RH ENGLAND
Titolo Testata:
PLANT SCIENCE
fascicolo: 1, volume: 97, anno: 1994,
pagine: 61 - 67
SICI:
0168-9452(1994)97:1<61:MDNSIE>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSGENIC PLANTS; GENE-EXPRESSION; PROTEIN; POLLEN;
Keywords:
METHANOL; ENDOGENOUS BETA-GLUCURONIDASE ACTIVITY; TRANSGENIC PLANTS; PLANT GENE EXPRESSION; NICOTIANA TABACUM; GUS A GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
11
Recensione:
Indirizzi per estratti:
Citazione:
J.E. Wilkinson et al., "METHANOL DOES NOT SPECIFICALLY INHIBIT ENDOGENOUS BETA-GLUCURONIDASE (GUS) ACTIVITY", PLANT SCI, 97(1), 1994, pp. 61-67

Abstract

One of the problems associated with the use of the bacterial gus A gene as a reporter is the presence of 'endogenous GUS' activity in some plant species. A recent paper [1] suggests that the addition of methanol to GUS reaction buffer specifically inhibits endogenous GUS activity while enhancing the activity of an introduced gus A gene. The results described in this paper suggest this phenomenon may be explained by the effect methanol has on cell membranes and demonstrate that it doesnot specifically inhibit endogenous GUS activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 04:35:11