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Titolo:
ANTIGENIC, STRUCTURAL, AND FUNCTIONAL-RELATIONSHIPS BETWEEN FIMBRIAE AND THE HEMAGGLUTINATING ADHESIN HA-AG2 OF PORPHYROMONAS-GINGIVALIS
Autore:
CHANDAD F; MOUTON C;
Indirizzi:
UNIV LAVAL,FAC MED DENT,RECH ECOL BUCCALE GRP LAVAL PQ G1K 7P4 CANADA UNIV LAVAL,FAC MED DENT,RECH ECOL BUCCALE GRP LAVAL PQ G1K 7P4 CANADA
Titolo Testata:
Infection and immunity
fascicolo: 12, volume: 63, anno: 1995,
pagine: 4755 - 4763
SICI:
0019-9567(1995)63:12<4755:ASAFBF>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTEROIDES-GINGIVALIS; ESCHERICHIA-COLI; MONOCLONAL-ANTIBODIES; EPITHELIAL-CELLS; MELANINOGENICUS; PURIFICATION; PROTEIN; ASACCHAROLYTICUS; IDENTIFICATION; ADHERENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
F. Chandad e C. Mouton, "ANTIGENIC, STRUCTURAL, AND FUNCTIONAL-RELATIONSHIPS BETWEEN FIMBRIAE AND THE HEMAGGLUTINATING ADHESIN HA-AG2 OF PORPHYROMONAS-GINGIVALIS", Infection and immunity, 63(12), 1995, pp. 4755-4763

Abstract

While the adhesive properties of Porphyromonas gingivalis are known to allow colonization of the subgingival tissues, the roles of fimbriaeand adhesin molecules in hemagglutination remain unclear, The purposeof this study was to analyze the antigenic, structural, and functional relationships of these two components, Five populations of monoclonal antibodies were produced against (i) the hemagglutinating adhesin HA-Ag2 resolved by crossed immunoelectrophoresis (CIE), (ii) native fimbriae, and (iii) each of the three immunoprecipitates, Ag8a, Ag8b, and Ag8c, that define fimbriae by CIE, The tests used for characterizationof the monoclonal antibodies included immunoblot reactivity, inhibition of hemagglutination, capacity to dissociate immunoprecipitates by CIE, localization of recognized epitopes by immunoelectron microscopy, and epitope mapping by competition enzyme linked immunosorbent assay, The results from the different immunochemical tests clearly showed a close antigenic relationship between fimbriae and the hemagglutinating adhesin HA-Ag2. We were able to establish that the epitopic domain H-1of HA-Ag2 is hemagglutinin specific and that domain F-2 is fimbria specific, Our data indicate that the polymeric structural unit of fimbriae must be complexed to HA-Ag2, the adhesin, to confer hemagglutination activity to the bacterial cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:51:06