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Titolo:
BINDING OF PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDES TO BASIC FIBROBLASTGROWTH-FACTOR, RECOMBINANT SOLUBLE CD4, LAMININ AND FIBRONECTIN IS P-CHIRALITY INDEPENDENT
Autore:
BENIMETSKAYA L; TONKINSON JL; KOZIOLKIEWICZ M; KARWOWSKI B; GUGA P; ZELTSER R; STEC W; STEIN CA;
Indirizzi:
COLUMBIA UNIV COLL PHYS & SURG,630 W 168TH ST NEW YORK NY 10032 COLUMBIA UNIV COLL PHYS & SURG NEW YORK NY 10032 POLISH ACAD SCI,CTR MOLEC & MACROMOLEC STUDIES PL-90363 LODZ POLAND
Titolo Testata:
Nucleic acids research
fascicolo: 21, volume: 23, anno: 1995,
pagine: 4239 - 4245
SICI:
0305-1048(1995)23:21<4239:BOPOTB>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
OLIGONUCLEOTIDES; AGENTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
L. Benimetskaya et al., "BINDING OF PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDES TO BASIC FIBROBLASTGROWTH-FACTOR, RECOMBINANT SOLUBLE CD4, LAMININ AND FIBRONECTIN IS P-CHIRALITY INDEPENDENT", Nucleic acids research, 23(21), 1995, pp. 4239-4245

Abstract

Antisense oligodeoxynucleotides can selectively inhibit the expression of individual genes and thus have potential applications in anticancer and antiviral therapy, A critical prerequisite to their use as therapeutic agents is the understanding of their non-specific interactionswith biological structures, e.g. proteins, In this study we examined the interactions of P-chiral phosphorothioate oligodeoxynucleotides with several proteins, The Rp- and Sp- diastereomers, and racemic machine-made mixtures, or M-oligodeoxynucleotides were used independently ascompetitors of the binding of a probe, phosphodiester oligodeoxynucleotide bearing a 5' alkylating moiety, to rsCD4, bFGF and laminin, These oligodeoxynucleotides were also used as competitors of the binding of a non-alkylating probe M-phosphorothioate oligodeoxynucleotide, 5'-P-32-SdT(18) to fibronectin, The average values of and quantitative estimates for the IC50 of competition and the constant of competition (K-c) of Rp-, Sp- and M- stereoisomers of several homo- and heteropolymeroligodeoxynucleotides were determined and compared, Surprisingly, in the proteins we studied, the values of IC50 and K-c for the Rp-, Sp- and M-oligodeoxynucleotides were essentially identical, Thus, the ability of the phosphorethioate oligodeoxynucleotides we employed, to bind to the proteins studied in this work, is virtually independent of P-chirality, Our results also imply that the role of the purine and pyrimidine bases in oligodeoxynucleotide-protein interactions, as well as the nature of the contact points (sulfur versus oxygen) between the oligomer and the protein, may be relatively unimportant.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 10:03:18