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Titolo:
P1 CLONES FROM DROSOPHILA-MELANOGASTER AS MARKERS TO STUDY THE CHROMOSOMAL EVOLUTION OF MULLERS-A-ELEMENT IN 2 SPECIES OF THE OBSCURA GROUPOF DROSOPHILA
Autore:
SEGARRA C; LOZOVSKAYA ER; RIBO G; AGUADE M; HARTL DL;
Indirizzi:
UNIV BARCELONA,FAC BIOL,DEPT GENET,DIAGONAL 645 E-08071 BARCELONA SPAIN HARVARD UNIV,DEPT ORGANISM & EVOLUT BIOL CAMBRIDGE MA 02138 CSIC,CTR INVEST DESENVOLUPAMENT E-08036 BARCELONA SPAIN
Titolo Testata:
Chromosoma
fascicolo: 2, volume: 104, anno: 1995,
pagine: 129 - 136
SICI:
0009-5915(1995)104:2<129:PCFDAM>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
YEAST ARTIFICIAL CHROMOSOMES; GENOME; PSEUDOOBSCURA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
C. Segarra et al., "P1 CLONES FROM DROSOPHILA-MELANOGASTER AS MARKERS TO STUDY THE CHROMOSOMAL EVOLUTION OF MULLERS-A-ELEMENT IN 2 SPECIES OF THE OBSCURA GROUPOF DROSOPHILA", Chromosoma, 104(2), 1995, pp. 129-136

Abstract

Thirty P1 clones from the X chromosome (Muller's A element) of Drosophila melanogaster were cross-hybridized in situ to Drosophila subobscura and Drosophila pseudoobscura polytene chromosomes. An additional recombinant phage lambda Dsuby was also used as a marker. Twenty-three (77%) of the P1 clones gave positive hybridization on D. pseudoobscura chromosomes but only 16 (53%) did so with those of D. subobscura. Eight P1 clones gave more than one hybridization signal on D, pseudoobscura and/or D, subobscura chromosomes. All P1 clones and lambda Dsuby hybridized on Muller's A element (X chromosome) of D. subobscura. In contrast, only 18 P1 clones and lambda Dsuby hybridized on Muller's A element (XL chromosomal arm) of D. pseudoobscura; 4 additional P1 clones hybridized on Muller's D element (XR chromosomal arm) of this species and the remaining P1 clone gave one hybridization signal on each arm ofthe X chromosome. This latter clone may contain one breakpoint of a pericentric inversion that may account for the interchange of genetic material between Muller's A and D elements in D. pseudoobscura. In contrast to the rare interchange of genetic material between chromosomal elements, profound differences in the order and spacing of markers weredetected between D. melanogaster, D. pseudoobscura and D. subobscura. In fact, the number of chromosomal segments delimited by identical markers and conserved between pairwise comparisons is small. Therefore, extensive reorganization within Muller's A element has been produced during the divergence of the three species. Rough estimates of the number of cytologically detectable inversions contributing to differentiation of Muller's A element were obtained. The most reliable of these estimates is that obtained from the D, pseudoobscura and D. melanogastercomparison since a greater number of markers have been mapped in bothspecies. Tentatively, one inversion breakpoint about every 200 kb hasbeen produced and fixed during the divergence of D. pseudoobscura andD. melanogaster.

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Documento generato il 04/12/20 alle ore 12:04:19